Molecular differentiation and determination of multi-drug resistant isolates of Pseudomonas species collected from burn patients in Kurdistan Region, Iraq

Narmin Saeed Merza; Rana Adel Hanoon; Haval Mohammed Khalid; Marwan Khalil Qader; Jaladet M.S. Jubrael

doi:10.15218/zjms.2018.051

Authors

Narmin Saeed Merza Department of Biology, College of Science, University of Duhok, Duhok, Iraq.
Rana Adel Hanoon Scientific Research Center, College of Science, University of Duhok, Duhok, Iraq.
Haval Mohammed Khalid Department of Biology, College of Science, University of Zakho, Duhok, Iraq.
Marwan Khalil Qader Department of Biology, College of Science, University of Duhok, Duhok, Iraq.
Jaladet M.S. Jubrael Scientific Research Center, College of Science, University of Duhok, Duhok, Iraq.

DOI:

https://doi.org/10.15218/zjms.2018.051

Keywords:

Pseudomonas aeruginosa, GurB2, Burn patients, Multi-drug resistance, gyr B2; Burn patients

Abstract

Background and objective: Pseudomonas aeruginosa is very a well-documented nosocomial and opportunistic microorganism, a little challenge is being present with the identiﬁcation of such pathogen. This study aimed to identify Pseudomonas on genus and species levels by conventional PCR and determine multi-drug resistant isolates.

Methods: A total of 180 clinical isolates of Pseudomonas species were recovered from in and outpatients who attended Azadi and Rezgari Teaching hospitals in Duhok and Erbil city from October 2015 to May 2016. These isolates were phenotypically identified using standard microbiological procedures. A total of 100 isolates were randomly selected and confirmed at a molecular level as Pseudomonas spp.

Results: By applying genus-specific gyr B2 primer which produced1130bp amplification band and sixty-eight isolates were identified by PCR as P. aueroginosa using species-specific primer for 16S rRNA region which showed 956bpamplicon. Forty-six isolates out of the sixty-eight resembling Pseudomonas aeruginosa were diagnosed as being multi-drug resistant isolates by the disc diffusion method.

Conclusion: It can be concluded that multi-drug resistant isolates can pose a serious threat for the hospital-resident patients as increasing numbers of these isolates are being recorded in local settings.

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