Histopathological and Immunohistochemical Approach for Characterization of Malignant Round Cell Tumors
Keywords:
Malignant round cell tumor, immunohistochemistryAbstract
Background and Objectives: To analyze the role of immunohistochemistry in characterization of malignant round cell tumors
Patients and Methods: Immunohistochemical staining (IHC) with (monoclonal or polyclonal) antibodies was performed on 127 cancer cases reported as malignant round cell tumors over a 12 month period from July 2008 through August 2009. Setting: Department of histopathology, Duhok Central Labs
Results: Malignant round cell tumors were more frequently located in the respiratory tract 30 (23.6%) followed by gastrointestinal tract 25 (19.7%), lymph node 19 (14.9%), and bone/soft tissue 19 (14.9%). Among these, 75 (82.7%) cases were primary and 22 (17.3%) metastatic. Application of immunohistochemistry resulted in characterization of 112 (88.2%) cases. Non-Hodgkin lymphoma 21 (16.5%) was at the top of the diagnosed list followed by adenocarcinoma 20 (15.7%), sarcoma 17 (13.4%), and small undifferentiated carcinoma 15 (11.8%).
Conclusions: Immunohistochemistry is a very helpful tool for characterization of malignant round cell tumors.
Metrics
References
Gatter KC, Alcock C, Heryet A, Pulford KA, Heyderman E, Taylor – Papadimitriou J, Stein H, and Mason DY. The differential diagnosis of routinely processed anaplastic tumors using monoclonal antibodies. Am J Clin Path. 1984;82:33-43.
Delellis RA and Dayal Y. The role of immunohistochemistry in the diagnosis of poorly differentiated malignant neoplasms. Semin Oncol 1987;14:173-192.
Sebire NJ, Gibson S, Rampling D, Williams S, Malone M, and Ramsay AD: Immunohistochemical findings in embryonal small round cell tumors with molecular diagnostic confirmation. Appl immunohistochem Mol Morphol. 2005;13:1-6.
Halliday BE, Slagel DD, Elsheikh TE, and Silverman JF. Diagnostic utility of MIC-2 immunocytochemical staining in the differential diagnosis of small blue cell tumors. Diagn Cytopathol. 1998;19:410-6.
Katz RL, Quezado M, and Senderowicz AM. An intraabdominal round cell neoplasm with features of primitive neuroectodermal and desmoplastic round cell tumor and a EWS/FLI-1 fusion transcript. Hum Pathol. 1997;28:502-9.
Ambros IM, Ambros PF, Strehl S, Kovar H, Gadner H, and Salzer-Kuntschik M. MIC2 is a specific marker for Ewing’s Sarcoma and peripheral primitive neuroectodermal tumors. Cancer. 1991;67:1886-1893.
Lakhtakia CR and Nema BSK. Immunophenotyping of tumours. MJAFI 2008;64:16-20.
Ahmad Z, Azad NS, and Bhurgari Y. Significance of immunohistochemistry in accurate characterization of malignant tumors. www.toodoc.com/Immunohistochemistry-ebook.html.
Chan JK. Advances in immunohistochemistry: impact on surgical pathology practice. Semin. Diagn. Pathol. 2000;17:170-177.
Slapak CA and Kufe DW. Principles of cancer therapy. In Isselbacher KJ, Braunvald E, Wilson JD, Martin JB, Fauci AS, Kasper DL eds: Harrison’s Principles of Internal Medicine, 13th Ed. Vol 2, 1994; McGraw-Hill, Inc. 1826-1840.
Raab SS. Cost effectiveness analysis in pathology. Clin. Lab. Med. 1999;19:757-71.
Special techniques in Surgical Pathology. In Rosai J, 9th Ed: Vol 2, Rosai and Ackerman’s Surgical Pathology. New York, Mosby. 2004. 45-63.
Taylor CR. The total test approach to standardization of immunohistochemistry. Arch. Pathol. Lab. Med. 2000;124:945-51.
Larsson L. Tissue preparation methods for light microscopic immunohistochemistry. Appl. Immunohistochem. 1993;1:2-16.
Hsu SM, Raine L, and Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 1981;29:577-80.
Shi SR, Cote RJ, and Taylor CR. Antigen retrieval techniques: current perspectives. J. Histochem. Cytochem. 2001;49:931-7.
Chan JKC, Banks PM, Cleary ML, Delsol G, De Wolf-Peeters C, Falini B, and Gatter KC. A Revised European–American Classification of lymphoid neoplasms proposed by the International Lymphoma Study Group. A summary version. Am J Clin Pathol 1995;103:543-560.
Jaffe ES, Harris NL, Stein H, and Vardiman JW. Tumors of hematopoitic and lymphoid tissues, pathology and genetics. World Health Organization classification of tumors, Lyon IARC Press, 2001.
Bianchini WA, Altemani AM, and Paschoal JR. Undifferentiated head and neck tumors: the contribution of immunohistochemical technique to differential diagnosis. Sao Paulo Med J 2003; 121(6):244-247.
Coindre JM, Tanguy F, Merlfo JP, De Mascarel I, De Mascarel A, and Trojani M. The value of immunohistological techniques in undifferentiated cancers. Tumori. 1986;72:539-44.
Gatter KC, Alcock C, Heryet A, and Mason DY. Clinical importance of analyzing malignant tumours of uncertain origin with immunohistological techniques. Lancet. 1985;1:1302-5.
Kleihues P, Davis RL, Ohgaki H, Burger PC, Westphal MM, and Cavanee WK. Diffuse astrocytoma. In Kleihues P, Cavanee W (eds): World Health Organization classification of tumors. Pathology and genetics – tumors of the nervous system. 2000; lyon, IARC Press, 22-26.
Johnson DE, Herndier BG, Medeiros LJ, Warnke RA, and Rouse RV. The diagnostic utility of the keratin profiles of hepatocellular carcinoma and cholangiocarcinoma. Am J Surg Pathol. 1988;12:187-197.
Papsidero LD, Croghan CA, Asirwattham J, Gaeta J, Abenoza P, Englander L, and Valenzuela L. Immunohistochemical demonstration of Prostate Specific Antigen in metastases with the use of monoclonal antibody. Am J Pathol. 1985;121:451- 454.
Frierson HF, Mills S, Fechner R, etal. Sinonasal undifferentiated carcinoma: an aggressive neoplasm derived from schneiderian epithelium and distinct from olfactory neuroblastoma. AmJSurgPathol. 1986;10:771-779.
Yaziji H and Gown AM. Immunohistochemical markers of melanocytic tumors. Int J Surg Pathol. 2003;11:11-15.
Ordonez NG. Value of cytokeratin 5/6 immunostaining in distinguishing epithelial mesothelioma of the pleura from lung adenocarcinoma. Am J Surg Pathol. 1998;22:1215-1221.
Downloads
Published
How to Cite
Issue
Section
License
The copyright on any article published in Zanco J Med Sci is retained by the author(s) in agreement with the Creative Commons Attribution Non-Commercial ShareAlike License (CC BY-NC-SA 4.0).
