Experimental Trial with Glutathione S Transferase as Vaccine Candidate against Hydatid Disease
Keywords:
Hydatid disease, Vaccination, Glutathione S transferaseAbstract
Background and Objective: Hydatid diseaseais is one of the most important zoonosis in the world. Because of economical and hygienic importance of the disease and no currently available drugs totally effective against it, researches in preventive methods in endemic areas are considerable. This study was undertaken to investigate the effectiveness of native protoscoleces glutathione S transferase as vaccine candidate against hydatid disease in mice.
Methods: Glutathione S transferase was partially purified from protoscoleces on sephadex G 150 column. Four groups of Balb/c mice (each of 20 animals) were immunized subcutaneously with 25µg, 50µg, 75µg and 100µg of the enzyme and three groups of mice were utilized as positive, negative and adjuvant controls.
Results: the study showed considerable protection (75%) and reduction (98.2%) in mice immunized with 75 µg of the enzyme. No significant differences were observed in total leukocyte number and in functions of the liver and kidney in all immunized groups as compared to control negative group. However, lymphocyte transformation response was highly significantly (P< 0.001) increased.
Conclusions: it can be concluded from the results, immunization of mice with 75µg of native protoscoleces glutathione S transferase conferred considerable protection against hydatid infection in mice.
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References
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