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Molecular detection of Nad5 in cystic echinococcosis in human and livestock animals in Erbil

Taban Ahmed Kwekha Qadir
Department of Medical Microbiology, College of Health Science, Hawler Medical University, Erbil, Kurdistan Region, Iraq
Hawri Mustafa Bakr
Department of Physiology and Microbiology, College of Medicine, Hawler Medical University, Erbil, Kurdistan Reign, Iraq.
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Copyright (c) 2025 Taban Ahmed Kwekha Qadir, Hawri Mustafa Bakr (Author)

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Abstract

Background and objective: Echinococcus granulosus can cause hydatidosis, or echinococcosis, in both herbivores and omnivores. Hydatid cysts are the larval stage of this tapeworm. As a zoonotic illness, it affects people all over the world. Devastating effects on human and animal health as well as massive economic losses, disproportionately felt in the agriculture sector, characterize this disease. Genomic segments from cox1 and nad1 genes have allowed for the identification of distinct genotypes of Echinococcus granulosus sensu stricto (s.s.) in both humans and domesticated animals. The current investigation aimed to distinguish between the G1/G3 genotypes of Echinococcus granulosus (s.s.) in cattle via the nad5 gene fragment.

Methods: The present study was conducted in Erbil city. Sheep, goat, and cattle hydatid cyst samples (36 isolates) were collected from the Erbil slaughterhouse. In addition, 11 fresh human hydatid cyst samples were taken from patients with hydatid cysts who underwent surgical operations in Rizgary Teaching Hospital. The hydatid cysts were subjected to examination for fertility and viability in addition to DNA extraction for genotyping based on the Nad5 gene.

Results: Out of 42 samples, 15 samples were sequenced, and all samples belonged to the G1 genotype (sheep strain). DNA sequencing of hydatid cysts from both animal and human sources showed mutations in various positions of the Nad5 gene, consistent with the dominant G1 genotype. In human samples, several variations overlap with other hosts, but unique ones include human 11,12 glycine/alanine at 142 arginine/glutamine, glycine/threonine at 303 valine/phenylalanine, T/A at 334 valine/aspartic acid, and T/C at 361 arginine/histidine, which result in 99% identity to the G1 strain.

Conclusion: Humans and animals in Erbil most commonly harbor the G1 genotype of E. granulosuss. s.s. according to the study. The findings provided additional evidence that the Nad5 gene accurately distinguished between humans and cattle, sheep, goats, and E.granulosus G1/G3 isolates.

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References

  1. Deplazes P, Rinaldi L, Rojas CA, Torgerson P, Harandi MF, Romig T, et al. Global distribution of alveolar and cystic echinococcosis. Adv Parasitol. 2017; 95:315-493 DOI: 10.1016/bs.apar.2016.11.001.
  2. Romig T, Deplazes P, Jenkins D, Giraudoux P, Massolo A, Craig PS, et al. Ecology and life cycle patterns of Echinococcus species. Adv Parasitol. 2017; 95:213-314 DOI: 10.1016/bs.apar.2016.11.002.
  3. Ali IF, Jihad TW. Perturbation of liver function markers and serum electrolytes associated with Echinococcus granulosus infection in sheep. Iraqi Journal of Veterinary Sciences. 2022; 36(1):65-9 DOI: 10.33899/ijvs.2021.128926.1624.
  4. Peruzzu A, Mastrandrea S, Fancellu A, Bonelli P, Muehlethaler K, Masala G, et al. Comparison and evaluation of analytic and diagnostic performances of four commercial kits for the detection of antibodies against Echinococcus granulosus and multilocularis in human sera. Comp Immunol Microbiol Infect Dis. 2022; 86:101816 DOI: 10.1016/j.cimid.2022.101816.
  5. Thompson R. Biology and systematics of Echinococcus. Adv Parasitol. 2017; 95:65-109. DOI: 10.1016/bs.apar.2016.07.001
  6. Laurimäe T, Kinkar L, Moks E, Romig T, Omer RA, Casulli A, et al. Molecular phylogeny based on six nuclear genes suggests that Echinococcus granulosus sensu lato genotypes G6/G7 and G8/G10 can be regarded as two distinct species. Parasitology. 2018; 145(14):1929-37 DOI: 10.1017/S0031182018000719.
  7. Barazesh A, Sarkari B, Sarısu G, Hami M, Mikaeili F, Aydin A, et al. Comparative Genotyping of Echinococcus granulosus Infecting Livestock in Turkey and Iran Türkiye ve İran'da Çiftlik Hayvanlarına Bulaşan Echinococcus granulosus’ un Karşılaştırmalı Genotiplenmesi. Turkiye Parazitoloji Dergisi. 2019; 43(3). https://doi.org/orcid.org/0000-0003-2045-9057.
  8. Barazesh A, Sarkari B, Shahabi S, Halidi AG, Ekici A, Aydemir S, et al. Genetic diversity of Echinococcus granulosus isolated from humans: A comparative study in two cystic echinococcosis endemic areas, Turkey and Iran. BioMed Research International. 2020; 2020(1):3054195 DOI: 10.1155/2020/3054195.
  9. Romig T, Ebi D, Wassermann M. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015; 213 (3-4):76-84. DOI: 10.1016/j.vetpar.2015.07.035.
  10. Kinkar L, Laurimäe T, Sharbatkhori M, Mirhendi H, Kia EB, Ponce-Gordo F, et al. New mitogenome and nuclear evidence on the phylogeny and taxonomy of the highly zoonotic tapeworm Echinococcus granulosus sensu stricto. Infect Genet Evol. 2017; 52:52-8. DOI: 10.1016/j.meegid.2017.04.023.
  11. Khademvatan S, Majidiani H, Foroutan M, Tappeh KH, Aryamand S, Khalkhali H. Echinococcus granulosus genotypes in Iran: a systematic review. J Helminthol. 2019; 93(2):131-8. DOI: 10.1017/S0022149X18000275.
  12. Alvi MA, Alshammari A, Ali RMA, Ul Haq S, Bashir R, Li L, et al. Revealing novel cytb and nad 5 genes-based population diversity and benzimidazole resistance in Echinococcus granulosus of bovine origin. Front Vet Sci. 2023; 10:1191271. DOI: 10.3389/fvets.2023.1191271.
  13. El-Bahy NM, Abdelaziz AR, Khalafalla RE. In-vitro evaluation of Nigella sativa and Punica granatum effect on protoscolices of hydatid cysts. Revista Brasileira de Parasitologia Veterinária. 2019; 28(2):210-14. DOI: 10.1590/S1984-29612019019
  14. Sharbatkhori M, Mirhendi H, Jex AR, Pangasa A, Campbell BE, Kia EB, et al. Genetic categorization of Echinococcus granulosus from humans and herbivorous hosts in Iran using an integrated mutation scanning‐phylogenetic approach. Electrophoresis. 2009; 30(15):2648-55.
  15. Karamian M, Haghighi F, Hemmati M. Genotyping of Cystic Hydatidosis Agents in Birjand, Eastern Iran. Modern Care Journal. 2017; 14(3). DOI: 10.5812/MODERNC.64531
  16. Cai Y, editor Analysis on gel electrophoresis in biology. E3S Web of Conferences; 2020: EDP Sciences.
  17. Kinkar L, Laurimäe T, Acosta-Jamett G, Andresiuk V, Balkaya I, Casulli A, et al. Distinguishing Echinococcus granulosus sensu stricto genotypes G1 and G3 with confidence: a practical guide. Infect Genet Evol. 2018; 64:178-84. DOI: 10.1016/j.meegid.2018.06.026
  18. Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, et al. Ovicidal potential of five different essential oils to control gastrointestinal nematodes of sheep. Pak Vet J. 2020; 41(3):353-8. DOI: 10.29261/pakvetj/2021.026
  19. Alberfkani MI, Albarwary AJ, Jaafar GM, Zubair AI, Abdullah RY. Molecular characterization and phylogenetic analysis of cox 1 and its 1 gene fragments of Moniezia species isolated from sheep. Pak Vet J. 2022 DOI: https://doi.org/10.29261/pakvetj/2022.073.
  20. Al-Barwari S, Saeed I, Khalid W, Al-Harmni K. human hydatidosis in Arbil, N. Iraq. Med J Islamic World Acad Sci. 1991; 4(4):330-5.
  21. Nematdoost K, Ashrafi K, Majidi-Shad B, Kia EB, Zeinali A, Sharifdini M. Genetic characterization of Echinococcus granulosus sensu lato in livestock and human isolates from north of Iran indicates the presence of E. ortleppi in cattle. Acta Parasitol. 2021; 66:446-54 DOI: 10.1007/s11686-020-00293-0.
  22. Bonelli P, Dei Giudici S, Peruzzu A, Mura L, Santucciu C, Maestrale C, et al. Identification of Echinococcus granulosus genotypes G1 and G3 by SNPs genotyping assays. Pathogens. 2021; 10(2):125.
  23. Hammad SJ, Cavallero S, Milardi GL, Gabrielli S, Al-Nasiri FS. Molecular genotyping of Echinococcus granulosus in the North of Iraq. Vet Parasitol. 2018; 249:82-7. DOI: 10.1016/j.vetpar.2017.11.010.
  24. Farhood A, ALMayah S, Abou Turab M. The relationship between genetic diversity and fertility status of hydatid cysts of Echinococcus granulosus isolated from human and some intermediate hosts in Thi-Qar province/Iraq. Int J Heal Sci. 2022; 6(S6):6839-49. DOI: 10.53730/ijhs.v6nS6.11947.
  25. Hamoo R, Abdulraheem S. Nad1 gene analysis of Echinococcus granulosus from sheep in Aqrah city, Iraq. Iraqi Journal of Veterinary Sciences. 2019; 33(2):341-5 DOI: 10.33899/ijvs.2019.162965.
  26. Abdulla RG, Mageed SN, Obed CE, Jumaa JA. Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors. Iraqi Journal of Veterinary Sciences. 2020; 34(2):321-7. DOI: 10.33899/ijvs.2019.126036.1213.
  27. Shahabi S, Sarkari B, Barazesh A. Echinococcus granulosus sensu stricto G1 is the predominant genotype in human and livestock isolates from Turkey and Iran, based on mitochondrial nad 5 gene differentiation. Parasit Vectors. 2021; 14:1-6. DOI: 10.1186/s13071-021-04869-1.
  28. Farhood AT, Abdulzahra AI, Saood AI. Hydatid cysts strains identification by mitochondrial dehydrogenase NADH subunit5 isolated from cattle and buffalo host in Babylon governorate, Iraq. Iraqi Journal of Veterinary Sciences. 2024; 38(2):399-404. DOI: 10.33899/ijvs.2023.142564.3185.
  29. Moradi M, Meamar AR, Akhlaghi L, Roozbehani M, Razmjou E. Detection and genetic characterization of Echinococcus granulosus mitochondrial DNA in serum and formalin-fixed paraffin embedded cyst tissue samples of cystic echinococcosis patients. PLoS One. 2019; 14(10):e0224501. 10.1371/journal.pone.0224501
How to Cite
Ahmed Kwekha Qadir, T. ., & Mustafa Bakr, H. . (2025). Molecular detection of Nad5 in cystic echinococcosis in human and livestock animals in Erbil. Zanco Journal of Medical Sciences (Zanco J Med Sci), 29(3), 679–697. https://doi.org/10.15218/zjms.2025.073

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