Advance Search

Circulating microRNA-1, and IL-18 are associated with inflammation in patients with type 2 diabetes mellitus: A case control study

Lajan Q Rahman
Department of Physiology, Microbiology and Genetics, College of Medicine, Hawler Medical University, Erbil, Iraq.
Prof. Ruqaya Barzinji
Department of Physiology, Microbiology and Genetics, College of Medicine, Hawler Medical University, Erbil, Iraq.
Amin Aziz Bakir
Department of Physiology, Microbiology and Genetics, College of Medicine, Hawler Medical University, Erbil, Iraq.
Keywords:
Copyright and Licensing:

Copyright (c) 2025 Lajan Q Rahman, Prof. Ruqaya Barzinji , Amin Aziz Bakir (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Share:
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Background and objective: IL-18 and micRNA-1 can be differentially regulated in cardiomyopathy, nephropathy, and amputation that are among the many micro- and macrovascular issues known to be the complications of type 2 diabetes mellitus (T2DM). Thus, this study aims to detect the association of miRNA-1 and IL-18 with type 2 diabetes mellitus.

Methods: This study included 90 participants including 30 diabetic patients, 30 pre-diabetic patients and 30 healthy controls in Erbil City, Iraq between November 2021 and May 2022. Different parameters were assessed for each participant including levels interleukin-18, high sensitivity C-reactive protein, lipid profile, and miRNA-1 profiling. 200µL of fresh plasma was utilized for the extraction of microRNA, and the extracted RNA was subsequently transformed into cDNA. Utilizing quantitative Real-Time PCR, the miRNA profiling analysis was carried out.

Results: When comparing the mean age of the diabetic patients to the other studied groups, there was a statistically significant difference (P <0.05). Furthermore, there was a greater proportion of males, smokers, obese people, and people with hypertension among DM patients. When compared to the healthy controls, the concentration levels of IL-18 and hsC-reactive protein in both DM and pre-DM patients exhibited a substantial elevation (P <0.01). The expression of miRNA-1 was down-regulated in healthy controls and considerably up-regulated in both diabetes and pre-diabetic patients.

Conclusion: These findings demonstrated that T2DM patients had higher expression of miRNA-1 than did healthy persons. MiRNAs may be very important in the etiology of type 2 diabetes via inducing inflammation. More research is necessary to determine their implications as diagnostic and preventive biomarkers.

PDF
PDF

Metrics

Metrics Loading ...

References

  1. Lee MG, Jeong MH, Lee KH, Park KH, Sim DS, Yoon HJ, et al. Prognostic impact of diabetes mellitus and hypertension for mid-term outcome of patients with acute myocardial infarction who underwent percutaneous coronary intervention. J Cardiol. 2012; 60(4):257–63. https://doi.org/10.1007/5584_2020_481
  2. Karczewski KJ, Snyder MP. Integrative omics for health and disease. Nat Rev Genet. 2018; 19(5):299–310.
  3. Srijyothi L, Ponne S, Prathama T, Ashok C, Baluchamy S. Roles of non-coding RNAs in transcriptional regulation. Transcr Post-transcriptional Regul. 2018; 55. doi: 10.5772/intechopen.76125
  4. Valinezhad OA, Safaralizadeh R, Kazemzadeh-Bavili M. Mechanisms of miRNA-mediated gene regulation from common downregulation to mRNA-specific upregulation. Int J Genomics. 2014; 2014.
  5. Kozomara A, Griffiths-Jones S. miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res. 2014; 42(D1):D68–73. https://doi.org/10.1093/nar/gky1141
  6. Arvola R, Abshire E, Bohn J, Goldstrohm AC. Mechanisms of Post-transcriptional Gene Regulation. Post-transcriptional Mech Endocr Regul. 2016; 1–36.
  7. Deiuliis JA. MicroRNAs as regulators of metabolic disease: pathophysiologic significance and emerging role as biomarkers and therapeutics. Int J Obes. 2016; 40(1):88–101.
  8. Yasuda K, Nakanishi K, Tsutsui H. Interleukin-18 in health and disease. Int J Mol Sci. 2019; 20(3):649.
  9. Striz I, Brabcova E, Kolesar L, Sekerkova A. Cytokine networking of innate immunity cells: a potential target of therapy. Clin Sci. 2014; 126(9):593–612.
  10. Hameed I, Masoodi SR, Mir SA, Nabi M, Ghazanfar K, Ganai BA. Type 2 diabetes mellitus: from a metabolic disorder to an inflammatory condition. World J Diabetes. 2015; 6(4):598
  11. Kaur G, Lakshmi PVM, Rastogi A, Bhansali A, Jain S, Teerawattananon Y, et al. "Diagnostic accuracy of tests for type 2 diabetes and prediabetes: A systematic review and meta-analysis." PloS one 15.11 (2020): e0242415. doi:10.1371/journal.pone.0242415
  12. Banerjee J, Nema V, Dhas Y, Mishra N. Role of microRNAs in type 2 diabetes and associated vascular complications. Biochimie. 2017; 139:9–19.
  13. Anderson E, Durstine JL. Physical activity, exercise, and chronic diseases: A brief review. Sport Med Heal Sci. 2019; 1(1):3–10.
  14. Nigi L, Grieco GE, Ventriglia G, Brusco N, Mancarella F, Formichi C, et al. MicroRNAs as regulators of insulin signaling: research updates and potential therapeutic perspectives in type 2 diabetes. Int J Mol Sci. 2018; 19(12):3705. https://doi.org/10.3390/ijms19123705
  15. McArdle MA, Finucane OM, Connaughton RM, McMorrow AM, Roche HM. Mechanisms of obesity-induced inflammation and insulin resistance: insights into the emerging role of nutritional strategies. Front Endocrinol (Lausanne). 2013; 4:52.
  16. Tallent R. Cocoa, Postprandial Lipoproteins, And Inflammation in Type 2 Diabetes: A Secondary Data Analysis. University of Nevada, Las Vegas; 2018.
  17. Muzurović E, Stanković Z, Kovačević Z, Škrijelj BŠ, Mikhailidis DP. Inflammatory markers associated with diabetes mellitus–old and new players. Curr Pharm Des. 2021; 27(27):3020–35.
  18. Park MH, Kim DH, Lee EK, Kim ND, Im DS, Lee J, et al. Age-related inflammation and insulin resistance: a review of their intricate interdependency. Arch Pharm Res. 2014; 37:1507–14
  19. Lana JP, de Oliveira MC, Silveira AL, Yamada LTP, Costa KA, da Silva SV, et al. Role of IL-18 in adipose tissue remodeling and metabolic dysfunction. Int J Obes. 2024:1–9.
How to Cite
Qasim Rahman, L., Muhamad Al-Barzinji, R., & Bakir, A. A. (2025). Circulating microRNA-1, and IL-18 are associated with inflammation in patients with type 2 diabetes mellitus: A case control study. Zanco Journal of Medical Sciences (Zanco J Med Sci), 396–402. https://doi.org/10.15218/zjms.2025.042

Send mail to Author

Send Cancel