TY - JOUR AU - Thanoon, Shima Ghazi AU - Al-Refai, Ali Sultan AU - Kamal, Amera Kamal PY - 2013/08/01 Y2 - 2024/03/28 TI - Antibacterial effect and healing potential of nutmeg oil for chemically induced oral ulcerations in rabbits JF - Zanco Journal of Medical Sciences (Zanco J Med Sci) JA - Zanco J Med Sci VL - 17 IS - 2 SE - Original Articles DO - 10.15218/zjms.2013.0022 UR - https://zjms.hmu.edu.krd/index.php/zjms/article/view/318 SP - 393-399 AB - <p style="text-align: justify; text-justify: inter-ideograph;"><strong style="font-size: 12pt;"><span style="font-family: Arial;">Background and objective: </span></strong><span style="font-family: Arial; font-size: 12pt;">Myristica fragrans is an aromatic green </span><span style="font-family: Arial; font-size: 12pt;">tree usually growing to around 5-13 meters high,</span><span style="font-family: Arial; font-size: 12pt;"> and their seeds (nutmegs) is firm broadly ovoid. The objectives of the study were to find the concentration of nutmeg oil that has maximum antibacterial action against </span><span style="font-family: Arial; font-size: 12pt;"><em>Staphylococcus aureus</em> and to evaluate the healing potential of this concentration on a chemically induced oral ulceration in the rabbit’s cheek mucosa.</span></p><p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: Arial; font-size: 12pt;">Methods:</span></strong> <span style="font-family: Arial; font-size: 12pt;">Different concentrations of nutmeg oil were prepared and their antibacterial activity against </span><span style="font-family: Arial; font-size: 12pt;"><em>Staphylococcus aureus</em> was determined by disk diffusion method. The chemically induced oral ulcerations in rabbit’s cheek mucosa were achieved by ethanol, and then these ulcerations were irrigated with 0.5ml (5%) of nutmeg oil twice daily. Biopsies were taken after four and eight days of the experiment, processed and stained by hematoxylin and eosin.</span></p><p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: Arial; font-size: 12pt;">Results:</span></strong> <span style="font-family: Arial; font-size: 12pt;">The concentration 100% of nutmeg oil and the dimethyl sulfoxide; which was used as diluents for preparing different concentrations of nutmeg oil, showed no inhibition zone for </span><span style="font-family: Arial; font-size: 12pt;"><em>Staphylococcus aureus</em>. The concentration (5%) showed maximum inhibitory zone (16.8mm) for the micro-organism which was parallel to the effects of amoxicillin (positive control), both with highly significant action (P&lt;0.05). The histopathological pictures showed a delay in the healing process of oral ulcers in rabbits treated by this concentration.</span></p><p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: Arial; font-size: 12pt;">Conclusion: </span></strong><span style="font-family: Arial; font-size: 12pt;">The concentration of nutmeg oil (5%) has a good antibacterial action against </span><span style="font-family: Arial; font-size: 12pt;"><em>Staphylococcus aureus,</em> but it causes a delay in the healing process of oral ulcerations.</span><span style="font-family: 'Arial','sans-serif';">&nbsp;&nbsp; </span></p> ER -