@article{Ismael_2016, title={P57Kip2 immunostaining, a diagnostic marker in differentiating complete hydatidiform mole from its mimics}, volume={20}, url={https://zjms.hmu.edu.krd/index.php/zjms/article/view/123}, DOI={10.15218/zjms.2016.0026}, abstractNote={<p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">Background and objective: </span></strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">The distinction of hydatidiform mole from hydropic abortion remains a problem because of interobserver and intraobserver variability.</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> This study aimed to determine</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> the utility of p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> as a diagnostic marker in differentiating complete hydatidiform mole from its mimics. </span></p> <p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">Methods: </span></strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">A total of 97 formalin fixed paraffin embedded material including forty cases of complete hydatidiform mole, 36 cases of partial hydatidiform mole and 21 cases of hydropic abortion were selected randomly from the files of histopathology laboratory of Maternity Teaching Hospital in Erbil. The samples were reviewed by two pathologists, afterward; immunohistochemical staining was performed by using a p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> marker. We considered p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> positive only if nuclear p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> staining was identified in at least 10% or more of all in a tissue section.      </span></p> <p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">Results: </span></strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">Negative immunostaining was seen in 77.5% of </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">the complete hydatidiform mole</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> in both villous cytotrophoblast and stromal cells. In contrast, 86.1% of </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">partial hydatidiform mole</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> showed positive immunostaining for p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">. All cases of hydropic abortion 100% were positive for p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> immunostaining. In all gestations, p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> was strongly expressed in decidua which served as internal positive control. The concordance between the initial histological diagnosis and p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> immunostaining was statistically significant (</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"><em>P</em> <0.001).</span><span style="font-family: ’Arial’,’sans-serif’;">    </span></p> <p style="text-align: justify; text-justify: inter-ideograph;"><strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">Conclusions: </span></strong><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> immunostaining is a highly sensitive and specific marker for diagnosis and classification of </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">hydatidiform mole</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">. p57</span><sup><span style="font-size: 12pt; line-height: 115%; font-family: ’Arial’,’sans-serif’;">Kip2</span></sup><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;"> staining has the advantage of differentiating hydropic abortuses from </span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">the complete hydatidiform mole</span><span style="font-family: ’Arial’,’sans-serif’; font-size: 12pt;">.</span> <span style="font-family: ’Arial’,’sans-serif’;"> </span></p>}, number={2}, journal={Zanco Journal of Medical Sciences (Zanco J Med Sci)}, author={Ismael, Ava T.}, year={2016}, month={Aug.}, pages={1316–1322} }