Association of seropositivity of toxoplasmosis and type 2 diabetes among adult females in Erbil city
DOI:
https://doi.org/10.15218/zjms.2023.026Keywords:
Type 2 diabetes, Toxoplasma, ELISA, IgG, IgM, LeptinAbstract
Background and objective: Toxoplasma gondii is an important opportunistic parasite in immune-compromised individuals that causes toxoplasmosis. An intracellular pathogen could cause severe complications. Diabetes is a significant component that enhances the host's vulnerability and risk of acquiring a variety of infections.
The objectives of the current study are to reveal the relationships between toxoplasmosis and type 2 diabetes in addition to find out the most specific gene for the detection of Toxoplasma gondii.
Methods: In this case-control study, 201 blood samples were taken from type 2 diabetic women with 100 healthy women as control groups to detect anti-Toxoplasma IgG and IgM antibodies by performing latex agglutination test and Enzyme-Linked Immunosorbent Assay (ELISA). Detection of B1 and RE genes of Toxoplasma gondii among seropositive individuals by nested PCR and evaluation of leptin hormone in both groups were performed.
Results: Among 201 type 2 diabetic cases, 64 cases (32.0%) were seropositive for anti-Toxoplasma antibodies, with 15 samples (15%) among 100 healthy control group via latex agglutination test. ELISA performed for only latex positive samples and the results were 64 ((32%) IgG+, (0%) IgM+) patient group (15.0% IgG+, 0.0% IgM+) healthy group with the P-value 0.0001 which is highly significant. The results of nested PCR were (46.88%) (IgG+, IgM-) and (1.56%) (IgG+, IgM-) for B1 and RE genes, respectively. Increased levels of leptin hormone were observed in patients in compare to healthy controls.
Conclusion: Both Diabetes and the elderly increase the chance of vulnerability to get toxoplasmosis. Diabetes, toxoplasmosis and high body mass index results in elevation of leptin hormone that leads to diabetes. B1 gene is the specific gene for detection of T. gondii in our area.
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Khalili M, Mahami-Oskouei M, Shahbazi A, Safaiyan A, Mohammadzadeh-Gheshlaghi N and Mahami-Oskouei L. The correlation between serum levels of anti-Toxoplasma gondii antibodies and the risk of diabetes. Iran J Parasit 2018; 13(4):637–42.
Ozcelik S, Alim M and Ozpinar N. Detection of Toxoplasma gondii infection among diabetic patients in Turkey. Clin Epidem Glob Health 2020; 8(3):899–902. https://doi.org/10.1016/j.cegh.2020.02.020
Hemida MH, Shahat SR, Bayoumy AS and Mohammad KAH. Prevalence of toxoplasmosis among diabetic patients. Europ J Pharmaceut Med Res 2017; 4(11):137–40.
Li Y-X, Xin H, Zhang X-Y, Wei C-Y, Duan Y-H, Wang H-F, et al. Toxoplasma gondii infection in diabetes mellitus patients in China: seroprevalence, risk factors, and case-control studies. Bio Med Res Intern 2018; 2018:1–9. DOI: 10.1155/2018/4723739
Mousavi M, Saravani R, Modrek MJ, Shahrakipour M and Sekandarpour S. Detection of Toxoplasma gondii in diabetic patients using the nested PCR assay via RE and B1 genes. Jundishapur J Microbiol 2016; 9(2):1–6. DOI: 10.5812/jjm.29493
Helieh SO. Toxoplasmosis, pancreatitis, obesity and drug discovery. Panc Diso Therap 2014; 4(2):1–5.
Molan A-L and Ismail MH. Study the possible association between toxoplasmosis and diabetes mellitus in Iraq. World J Pharmaceut Sci 2017; 6:85–96.
Nihad SF and Hamad SS. Seroprevalence of Toxoplasma gondii infection in diabetic patients at Kirkuk province. IJSBAR 2017; 36:157–65.
Modrek MJ, Saravani R, Mousavi M, Khorashad AS and Piri M. Investigation of IgG and IgM antibodies against Toxoplasma gondii among diabetic patients. Int J Infect 2015; 2(3):1–5. DOI:10.17795/iji-27595
Han K, Shin D-W, Lee T-Y and Lee Y-H. Seroprevalence of Toxoplasma gondii infection and risk factors associated with seropositivity of pregnant women in Korea. J Parasit 2008; 94(4):963–5. DOI: 10.1645/GE-1435.1
Zemene E, Yewhalaw D, Abera S, Belay T, Samuel A and Zeynudin A. Seroprevalence of Toxoplasma gondii and associated risk factors among pregnant women in Jimma town, Southwestern Ethiopia. BMC Infect Dis 2012; 12(1):1‒6. DOI: 10.1186/1471-2334-12-337
Sarkari B, Shafiei R, Zare M, Sohrabpour S and Kasraian L. Seroprevalence and molecular diagnosis of Toxoplasma gondii infection among blood donors in southern Iran. J Infect Devel Count 2014; 8(4):543‒7. DOI: 10.3855/jidc.3831
Cardona N, Basto N, Parra B, Zea AF, Pardo CA, Bonelo A, et al. Detection of Toxoplasma DNA in the peripheral blood of HIV-positive patients with neuro-opportunistic infections by a real-time PCR assay. J Neuroinfect Dis 2011; 2(2011):1‒6. DOI: 10.4303/jnp/N110402
Rahimi Esboei B, Kazemi B, Zarei M, Mohebali M, Keshavarz Valian H, Shojaee S, et al. Evaluation of RE and B1 genes as targets for detection of Toxoplasma gondii by nested PCR in blood samples of patients with ocular toxoplasmosis. Acta Parasitologica 2019; 64(2):384‒389. DOI: 10.2478/s11686-019-00056-6
Wahab T, Edvinsson B, Palm D and Lindh J. Comparison of the AF146527 and B1 repeated elements, two real-time PCR targets used for detection of Toxoplasma gondii. J Clinic Microbiol 2010; 48(2):591‒2. DOI: 10.1128/JCM.01113-09
Sağlam T, Düşen S, Mete E and Karaman Ü. Comparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkey. Acta Parasitologica 2022; 67(1):555‒9. DOI: 10.1007/s11686-021-00494-1
Fallahi S, Kazemi B, Bandehpour M, Lasjerdi Z, Taghipour N, Zebardast N, et al. Comparison of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer. Parasit Intern 2014; 63(1):37‒41. DOI: 10.1016/j.parint.2013.08.005.
Hassan AJ, Mohammed NS, Shweash M and Hadeed HM. Impact of Anti-Toxoplasma gondii and adipose hormones with Insulin Resistant on obese aborted women. Internat J Pharmaceut Qual Assuran 2020; 10(1):114‒8. DOI: 10.25258/ijddt.v10i1.25
Yahya R, Awad S, Hussein D, Hamed E, Raida S, Yahya, et al. Leptin and Leptin Receptor Polymorphism in Egyptian Diabetic Children with Latent Toxoplasmosis. Intern J Scie Eng Res 2019; 10:296‒311.
Anandhan P. The Effect Of Exposure To Toxoplasma gondii Profilin On Leptin Level In Rattus Norvegicus Wistar Strain Rats Given Normal Diet And Hypercaloric Diet [Bacelore ]: Universitas Brawijaya; 2018.
Facey A, Dilworth L and Irving R. A review of the leptin hormone and the association with obesity and diabetes mellitus. J of Diabet Metab 2017; 8(3):1‒13. DOI: 10.4172/2155-6156.1000727
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