P63 expression profile in uterine cervical lesions


  • Yusra Abdulkhaliq Qasim Department of Pathology, College of Medicine, Hawler Medical University, Erbil, Iraq.




P63 immunostaining, Cervical squamous cell carcinoma


Background and objective: P63 is a member of the p53 gene family. It plays an essential role in the normal development of the cervix. This study aimed to assess p63 expression in different cervical lesions and the correlation between their expression in cervical intraepithelial neoplastic changes and invasive cervical cancer. 

Methods: A total of 62 cervical tissues were performed for p63 immunostaining, including 15 samples of non-neoplastic reactive cervicitis, 27 cervical intraepithelial neoplastic changes, and 15 invasive cervical cancer cases as well as five samples of normal cervical tissue as control.

Results: P63 in normal cervical squamous epithelium had restricted staining pattern in the nuclei of basal cells. Reactive cervicitis staining ranged from restricted staining pattern in basal cells to mild increase in staining intensity involving upper parabasal cells. P63 was positive in all CIN lesions, and staining intensity increased significantly with increasing severity of cervical intraepithelial lesions compared with non-neoplastic epithelium. All cervical squamous cell carcinoma had high staining intensity while adenocarcinoma and small cell carcinoma were stained negative.

Conclusion: P63 can be used as a marker for the distinction between reactive cervicitis and cervical intraepithelial changes. It is also of value for grading of CIN lesions and differentiation between squamous cell carcinoma, adenocarcinoma, and small cell carcinoma.


Metrics Loading ...


Koyuncuer A. Immunohistochemical expression of p63, p53 in urinary bladder carcinoma. Indian J Pathol Microbiol 2013; 56(1):10–5.

Inoue K, Fry, EA. Alterations of p63 and p73 in human cancers. SubcellBiochem2014; (85)17–40.

Abilasha R, Ramani P, Sherlin H J, Premkumar P, Natesan A. Immunohistochemical evaluation of oral epithelial dysplasia using cyclin-D1, p27 and p63 expression as predictors of malignant transformation. Nat Sci Biol Med 2013; 4(2):349–58.

Sharada P, Swaminathan U, Nagamalini B R, Vinodkumar K, Ashwini B K, Lavanya V. A Semi-quantitative analysis of immunohistochemical expression of p63, Ki-67, Cyclin-D1, and p16 in common oral potentially malignant disorders and oral squamous cell carcinoma. Journal of Dr. NTR University of Health Sciences 2018; 7(2):120–8.

Shetty S, Krishnapillai R, Prabhu S. Assessment and comparison of p53 and p63 expression in oral epithelial dysplasia and squamous cell carcinoma. SRM Journal of Research in Dental Sciences 2014; 5(3):149–54.

- Mohite DP, Hande AH, Gupta R, Chaudhary MS, Palit S, Gawandi M. Immunohistochemical evaluation of expression pattern of P53, P63 and P73 in epithelial dysplasia. Journal of Data Meghe Institute of Medical Science University 2018; 13(3):122–9.

Jacob AA, Sundaram A. P16, Ki67 and P63 staining pattern in squamous metaplasia, CIN and cervical cancer. Int J Res Med Sci 2018; 6(3):882.

Saritha VN, Veena VS, Krishna J, Somanathan T, Sujathan K. Significance of DNA Replication Licensing Proteins (MCM2, MCM5 and CDC6), p16 and p63 as Markers of Premalignant Lesions of the Uterine Cervix: Its Usefulness to Predict Malignant Potential. Asian Pacific Journal of Cancer Prevention 2017; 19(1):141–58.

Kurman RJ, Carcangiu ML, Herrington CS, Young RH. WHO Classification of tumours. 4thed. Lyon: WHO; 2014.

Martin CM, O’Leary JJ, Phil D. Histology of cervical intraepithelial neoplasia and the role of biomarkers. Best Pract Res Clin Obstetgynaecol 2011; 25:605–15.

Díaz CM G, Rincón AE S, Camacho G V, Vázquez G B, Quintana SR, Wentzensen N, et al. P16INK4a immunohistochemistry improves the reproducibility of the histological diagnosis of cervical intraepithelial neoplasia in cone biopsies. Gynecol Oncol 2008; 111:120–4.

Van Niekerk D, Guillaud M, Matisic J, Benedet JL, Freeberg Ja, Follen M, et al. P16 and MIB1 improve the sensitivity and specificity of the diagnosis of high grade squamous intraepithelial lesions: methodological issues in a report of 447 biopsies with consensus diagnosis and HPv HCII testing. Gynecol Oncol 2007; 107:233–40.

Lobato S, Tafuri A, Fernandes PÁ, Caliari Mv, Silva MX, Xavier Ma, et al. Maintenance 7 protein is a reliable biological marker for human cervical progressive disease. J Gynecol Oncol 2012; 23:11–5.

Mitildzans A, Arechvo A, Rezeberga D, Isajevs S. Expression of p63, p53 and Ki-67 in Patients with Cervical Intraepithelial Neoplasia. Turk Patoloji Derg 2017; 33:9–16.

Kim SU, Lee JU, Lee DW, Kim MJ, Lee HN. The prognostic significance of P16, KI 67, P63 and CK 17 expression determined by immunohistochemical staining in cervical intraepithelial I. Korean J Obstet Gynecol 2011; 54(4):184–91.

AbdRaboh NM, Hakim SA. Diagnostic role of DOG1 and p63 immunohistochemistry in salivary gland carcinomas. Int J Clin Exp Pathol 2015; 8(8):9214–22.

Romus I, Triningsih FE, Mangunsudirdjo S, Harijadi A. Clinicopathology significance of p53 and p63 expression in Indonesian cervical squamous cell carcinomas. J Cancer Prev 2013; 14(12):7737–41.

DasL,Naskar S, Sarkar T, Kumar A , Das S, Chatterjee J. Immunohistochemical evaluation of prime molecules in cervical lesions towards assessment of malignant potentiality. J Cancer Res Ther 2018; 14(2):377–81

KalofAN, Cooper K. Our approach to squamous intraepithelial lesions of the uterine cervix. J Clin Pathol 2007; 60(5):449–455.

Lin Z, Liu M, Li Z, Kim C, Lee E, Kim I. Protein expression in uterine cervical and endometrial cancers. J Cancer Res Clin Oncol 2006; 132:811–6.

Houghton OI, McCluggage WG.The expression and diagnostic utility of p63 in the female genital tract. Adv Anat Pathol 2009; 16(5):316–21.

Glenn W. Immunohistochemistry as a diagnostic aid in cervical pathology. Pathology 2007; 39(1):97–111.




How to Cite

Qasim, Y. A. (2020). P63 expression profile in uterine cervical lesions. Zanco Journal of Medical Sciences (Zanco J Med Sci), 24(2), 197–204. https://doi.org/10.15218/zjms.2020.023



Original Articles