The protective effect of Fumaria officinalis against the testicular toxicity of fluoxetine in rat

Abdalmuhaimn Y. Sharef; Falah M. Aziz; Aveen N. Adham

doi:10.15218/zjms.2020.015

Authors

Abdalmuhaimn Y. Sharef Department of Clinical Analysis, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
Falah M. Aziz Department of Biology, College of Science, Salahaddin University, Erbil, Iraq.
Aveen N. Adham Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq.

DOI:

https://doi.org/10.15218/zjms.2020.015

Keywords:

Fumaria officinalis, Fluoxetine, Seminiferous tubules, Erbil city

Abstract

Background and objective: Extracts of Fumaria species have been traditionally used for the treatment of some skin diseases, rheumatism, stomach ache, fever, and male infertility mainly because of the presence of isoquinoline alkaloids. On the other side, fluoxetine is used as an antidepressant induce sexual dysfunction and a decrease in sperm concentration, motility, and morphology. The present investigation dealt with the study of the protective role played by the Fumaria officinalis extract against the selective serotonin reuptake inhibitor (SSRI) induced testicular toxicity in the rat. Therefore, the purpose of this work was to determine the benefits of Fumaria officinalis extract in animals treated with fluoxetine.

Methods: Thirty six male rats were divided into three following groups, control group, fluoxetine (20 mg/kg/day) treated-group, and the same dose of Fluoxetine plus Fumaria officinalis extract (150 mg/kg/day) as a third group. Drugs and extract were administered orally for 30 days. Sections of testes were stained by Haematoxylin and Eosin.

Results: Fluoxetine has caused several structural changes in the rat testis such as vacuolation within germinal epithelium of seminiferous tubules, decrease the diameter of these tubules and decrease number of sertoli and germinal cells. Treating the fluoxetine exposed rats with Fumaria officinalis extract has shown to ameliorate the above histological changes.

Conclusion: Fumaria officinalis extract can protect testis from histological alterations caused by the toxicity of the fluoxetine.

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References

Sharma A. Anorexia nervosa and bulimia nervosa: An appraisal. Drugs Today (Barc) 2001; 37(4):229–36.

Vaswani M, Linda FK, Ramesh S. Role of selective serotonin reuptake inhibitors in psychiatric disorders: a comprehensive review. Prog Neuro psycho pharmacol Biol Psychiatry 2003; 27(1):85–102.

Mielniczuk L, DeKemp RA, Dennie C, Yoshinaga K, Burwash IG, Bénard F, et al. Images in cardiovascular medicine. Fluorine-18-labeled deoxyglucose positron emission tomography in the diagnosis and management of aortitis with pulmonary artery involvement. Circulation 2005; 111(22):375–6.

Ross JB. Drugs and the treatment of psychiatric disorders in depression and anxiety disorders. Goodman and Gillman’s. The pharmacological basis of therapeutics. 10th ed. New York: Mc Graw-Hill Companies; 2001. P. 447–83.

Silva JVA, Lins AMJ, Amorim AA, Pinto FC, Deiró TBJ, de Oliveira JRM, et al. Neonatal administration of fluoxetine decreased final sertoli cell number in Wistar rats. Int J Morphol 2008; 26(1):51–62.

Ortiz Á, Espino J, Bejarano I, Lozano GM, Monllor F, García JF, et al. The correlation between urinary 5-hydroxyindoleacetic acid and sperm quality in infertile men and rotating shift workers. Reprod Biol Endocrinol 2010; 8(1):138.

Bataineh HN, Daradka T. Effects of long-term use of fluoxetine on fertility parameters in adult male rats. Neuro Endocrinol Lett 2007; 28(3):321–5.

Hendrick V, Gitlin M, Altshuler L, Korenman S. Antidepressant medications, mood and male fertility. Psycho Neuro Endocrinol 2000; 25(1):37–51.

Jaberian H, Piri K, Nazari J. Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chem 2013; 136(1):237–44.

Mingli Z, Lidén M. Fumaria Linnaeus, Sp. Flora of China 2008; 7:428.

Patel A, Shenouda N, Tallis G. PDR for Herbal Medicines. 4th ed. Thomson Healthcare, Montvale; 2007.

Suau R, Cabezudo B, Rico R, Najera F, López‐Romero JM. Direct determination of alkaloid contents in Fumaria species by GC‐MS. Phytochemical Analysis. An Int J Plant Chem Biochem Tech 2002; 13(6):363–7.

Maiza-Benabdesselam F, Khentache S, Bougoffa K, Chibane M, Adach S, Chapeleur Y, et al. Antioxidant activities of alkaloid extracts of two Algerian species of Fumaria: Fumaria capreolata and Fumaria bastardii. Rec Nat Prol 2007; 1(2-3):28.

Mukhtar GZ, Chrioma BA, Zainab R, Muhmmad AI, Ibrahim Y, Yunusa I. Cinnamomun cassia, Ficus carica and Fumaria officinalis possesses aphrodisiac activity in male Wister rats. Ann Exp Bio 2015; 3(3):14–7.

Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009; 22(1):102–6.

Dorostghoal M, Seyyednejad SM, Khajehpour L, Jabari A. Effects of Fumaria parviflora leaves extract on reproductive parameters in adult male rats. Iran J Reprod Med 2013; 11(11):891.

Walia V, Gilhotra N. GABAergic influence in the antidepressant effect of fluoxetine in unstressed and stressed mice. J Appl Pharm Sci 2017; 7(03):141–7.

Silva Junior VA, Monteiro Filho O, Ferreira Pinto C, de Torres SM, Mendes Tenorio B. Testis evaluation of adult Wistar rats after neonatal treatment with fluoxetine. Acta Sci Biol Sci 2013; 35(1):115–22.

Erdemir F, Atilgan D, Firat F, Markoc F, Parlaktas BS, Sogut E. The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats. Int Braz J Urol 2014; 40(1):100–8.

Geetha U. Post treatment analysis of fluoxetine hydrochloride on testis of wistar rats. Int J STEM Educ 2017; 5(1):2321–9009.

National Toxicology Program. NTP-CERHR monograph on the potential human reproductive and developmental effects of fluoxetine. NTP CERHR MON 2004; (13):i–III24.

Nasrabadi MH, Aboutalebi H, Naseri M. Effect of Fumaria parviflora alcoholic extract on male rat’s reproductive system. J Med Plants Res 2012; 6(10):2004–10.

Aggarwal A, Jethani S L, Rohatgi R K, Kalra J. Effects of Fluoxetine on Testis of Albino rats. A Histological Assessment. Int J Sci Eng Res 2012; 3(7):1–5.

Ortega-Pacheco A, Rodríguez-Buenfil JC, Segura-Correa JC, Bolio-Gonzalez ME, Jiménez-Coello M, Linde Forsberg C. Pathological conditions of the reproductive organs of male stray dogs in the tropics: prevalence, risk factors, morphological findings and testosterone concentrations. Reprod Domest Anim 2006; 41(5):429–37.

Aggarwal A, Jethani S L, Rohatgi R K, Kalra J. Effect of Fluoxetine on epididymis of albino rats: A Histological study. Int J Sci Eng Res 2013; 4(8):1457–62.

Unnikrishnan G, Shivabalan R, Lillian J. Sub-acute toxicity of fluoxetine hydrochloride on fertility of male wistar rats. J Herb Med Toxicol 2009; 3:23–30.

Ivanov I, Vrancheva RZ, Marchev AS, Petkova NT, Aneva IY, Denev PP, et al. Antioxidant evaluation of their antioxidant and antibacterial activities, Industrial Crops and activities and phenolic compounds in Bulgarian Fumaria species. Int J Curr Microbiol App Sci 2014; 3:296–306.

Wasu SJ, Muley BP. Antioxidant activity of Fumaria officinalis Linn. and its study on ethanol induced–immunosupression. Res J Pharm Technol 2009; 2(2):405–8.

Khamtache-Abderrahim S, Lequart-Pillon M, Gontier E, Gaillard I, Pilard S, Mathiron D, et al. Isoquinoline alkaloid fractions of Fumaria officinalis: Characterization and evaluation of their antioxidant and antibacterial activities. Ind Crops Prod 2016; 30(94):1001–8.

Souri M, Heydari NM, shiravi A, Habibi Z. Interaction between Fumaria parviflora aqueous extract and cholorambocil on spermatogenesis in rats. J Animal Biol 2009; 2(1):11–8.