Effects of omega-3 on androgenic activity in cadmium-induced hypertensive rats
DOI:
https://doi.org/10.15218/zjms.2019.048Keywords:
Cadmium, Omega-3; Blood pressure, Sperm parameter, Male hormonesAbstract
Background and objective: Cadmium is a widely distributed metal in the environment that causes testicular toxicity and various tissue damage associated with hypertension. Omega-3, an essential fatty acid, has been demonstrated to be an effective antioxidant and anti-inflammatory agent. This study aimed to investigate the toxic effects of cadmium on cardiovascular and reproductive systems in male rats and examine the possible protective effects of omega-3 against cadmium-induced hypertension and testicular dysfunctions.
Methods: The rats were divided into three groups. The first group served as the control, the second group received cadmium chloride in drinking water, and the third group received cadmium chloride plus omega-3 for 28 days. Blood pressure and heart rate were estimated. Blood samples were collected; sexual hormones and other biochemical parameters were evaluated. Testes and epididymis were taken for semen analysis and histopathology.
Results: Significant adverse effects were seen in the studied parameters of the cardiovascular and reproductive systems after cadmium exposure, including hypertension, alteration in sperm parameters, weights of sex glands, and histological structures. In contrast, co-administration of omega-3 significantly reduced mean blood pressure, but improved sperm parameters and the histology of testis. No protective effect of omega-3 could be seen on male hormone and lipid profile parameters except high-density lipoprotein.
Conclusion: Exposure to cadmium induced hypertension, impaired testicular functions, and histology. Omega-3 has protective effects on vascular and testicular functions. Therefore, supplementation with omega-3 had a protective effect against cardiovascular and reproductive toxicity induced by cadmium.
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Marettová E, Maretta M, Legáth J. Toxic effects of cadmium on testis of birds and mammals: a review. Anim Reprod Sci 2015; 155:1–10.
Sarkar AN, Ravindran GE, Krishnamurthy VI. A brief review on the effect of cadmium toxicity: from cellular to organ level. Int J Biotechnol Res 2013; 3(1):17–36.
Oliveira TF, Batista PR, Leal MA, Campagnaro BP, Nogueira BV, Vassallo DV, et al. Chronic Cadmium Exposure Accelerates the Development of Atherosclerosis and Induces Vascular Dysfunction in the Aorta of ApoE−/− Mice. Biol Trace Elem Res 2018; 29:1–9.
da Cunha Martins A, Carneiro MF, Grotto D, Adeyemi JA, Barbosa F. Arsenic, cadmium, and mercury-induced hypertension: mechanisms and epidemiological findings. J Toxicol Environ Health, Part B 2018; 21(2):61–82.
Asgary S, Movahedian A, Keshvari M, Taleghani M, Sahebkar A, Sarrafzadegan N. Serum levels of lead, mercury and cadmium in relation to coronary artery disease in the elderly: A cross-sectional study. Chemosphere 2017; 180:540–4.
Djukić-Ćosić D, Jovanović MĆ, Bulat ZP, Ninković M, Maličević Ž, Matović V. Relation between lipid peroxidation and iron concentration in mouse liver after acute and subacute cadmium intoxication. J Trace Elem Med Biol 2008; 22(1):66–72.
Manna P, Sinha M, Sil PC. Cadmium induced testicular pathophysiology: prophylactic role of taurine. Reprod Toxicol 2008; 26(3-4):282–91.
Thompson J, Bannigan J. Cadmium: toxic effects on the reproductive system and the embryo. Reprod Toxicol 2008; 25(3):304–15.
Djuric A, Begic A, Gobeljic B, Stanojevic I, Ninkovic M, Vojvodic D, et al. Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. Food Chem Toxicol 2015; 86:25–33.
Lamas CD, CuquettoLeite L, do Nascimento SE, Thomazini BF, Cordeiro GD, Predes FD, et al. Grape juice concentrate alleviates epididymis and sperm damage in cadmium intoxicated rats. Int J Clin Exp Pathol 2017; 98(2):86–99.
Wang L, Li Y, Fu J, Zhen L, Zhao N, Yang Q, et al. cadmium inhibits mouse sperm motility through inducing tyrosine phosphorylation in a specific subset of proteins. Reprod Toxicol 2016; 63:96–106.
Lacorte LM, Seiva FR, Rinaldi JC, Delella FK, Moroz A, Sarobo C, et al. caffeine reduces cadmium accumulation in the organism and enhances the levels of antioxidant protein expression in the epididymis. Reprod Toxicol 2013; 35:137–43.
Ismail TF, Aziz FM. The protective effect of omega-3 oil against the hepatotoxicity of cadmium chloride in adult and weanling rats. In AIP Conference Proceedings 2017 Sep 21 (Vol. 1888, No. 1, p. 020027). AIP Publishing.
O’Mahoney LL, Matu J, Price OJ, Birch KM, Ajjan RA, Farrar D, et al. Omega-3 polyunsaturated fatty acids favourably modulate cardiometabolic biomarkers in type 2 diabetes: a meta-analysis and meta-regression of randomized controlled trials. Cardiovasc Diabetol 2018; 17(1):98.
Ozen OA, Cosar M, Sahin O, Fidan H, Eser O, Mollaoglu H, et al. The protective effect of fish n-3 fatty acids on cerebral ischemia in rat prefrontal cortex. J Neurol Sci 2008; 29(3):147.
Siegert E, Paul F, Rothe M, Weylandt KH. The effect of omega-3 fatty acids on central nervous system remyelination in fat-1 mice. BMC Neurosci 2017; 18(1):19.
Uygur R, Aktas C, Tulubas F, Uygur E, Kanter M, Erboga M, et al. Protective effects of fish omega 3 fatty acids on doxorubicin-induced testicular apoptosis and oxidative damage in rats. Andrologia 2014; 46(8):917–26.
Zhang J, Wang Y, Fu L, Feng YJ, Ji YL, Wang H, Xu DX. Subchronic cadmium exposure upregulates the mRNA level of genes associated to hepatic lipid metabolism in adult female CD1 mice. J Appl Toxicol 2018; 38(7):1026–35.
Nagahama H, Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Namioka T, Namioka A, et al. Preservation of interhemispheric cortical connections through corpus callosum following intravenous infusion of mesenchymal stem cells in a rat model of cerebral infarction. Brain Res 2018; 1695:37–44.
Shabanian S, Farahbod F, Rafieian M, Ganji F, Adib A. The effects of Vitamin C on sperm quality parameters in laboratory rats following long-term exposure to cyclophosphamide. J Adv Pharm Technol Res 2017; 8(2):73.
Yucra S, Gasco M, Rubio J, Nieto J, Gonzales GF. Effect of different fractions from hydroalcoholic extract of Black Maca (Lepidiummeyenii) on testicular function in adult male rats. Fertil Steril 2008; 89(5):1461–7.
Ellenburg J, Drwiega J, Posey A, Goldberg M, Gordetsky J, Kolettis P. MP19-18 The use of formalin as a fixative for testicular biopsies. J Urol 2018; 199 (4):250–1.
Battan G, Tandon R, Vasenwala SM, Faruqi NA. Effect of methotrexate on testis. An experimental study on Albino rat. Annals of Interventional Medical and Dental Research. 2015; 1(3):170–4.
Broseghini-Filho GB, Almenara CC, Vescovi MV, Faria TD, Vassallo DV, Angeli JK, et al. Acute cadmium exposure reduces the local angiotensin I converting enzyme activity and increases the tissue metal content. Biol Trace Elem Res 2015; 166(2):149–56.
Angeli JK, Pereira CA, de Oliveira FT, Stefanon I, Padilha AS, Vassallo DV. Cadmium exposure induces vascular injury due to endothelial oxidative stress: the role of local angiotensin II and COX-2. Free Radic Biol Med 2013; 65:838–48.
Qi X, Qin Z, Tang J, Han P, Xing Q, Wang K, et al. Omega-3 polyunsaturated fatty acids ameliorates testicular ischemia-reperfusion injury through the induction of Nrf2 and inhibition of NF-κB in rats. Exp Mol Pathol 2017;103(1):44–50.
Bini G, Santini G, Chelazzi G. Pre-exposure to cadmium or zinc alters the heart rate response of the Crayfish procambarus clarkia towards copper. Bull Environ Contam Toxicol 2015; 95(1):12–7.
McGinnis WR, Audhya T, Edelson SM. Proposed toxic and hypoxic impairment of a brainstem locus in autism. Int J Environ Res Public Health 2013; 10(12):6955–7000.
Murugavel P, Pari L. Diallyltetrasulfide protects cadmium-induced alterations in lipids and plasma lipoproteins in rats. Nutr Res 2007; 27(6):356–61.
Larregle EV, Varas SM, Oliveros LB, Martinez LD, Antón R, Marchevsky E, et al. Lipid metabolism in liver of rat exposed to cadmium. Food Chem Toxicol 2008; 46(5):1786–92.
Samarghandian S, Azimi-Nezhad M, Shabestari MM, Azad FJ, Farkhondeh T, Bafandeh F. Effect of chronic exposure to cadmium on serum lipid, lipoprotein and oxidative stress indices in male rats. Interdiscip Toxicol 2015; 8(3):151–4.
Nourbakhsh M, Nourbakhsh M, Azar MR. The effect of n-3 PUFA on lipid profile, inflammation and insulin resistance. Endocrine Abstracts 2018; 56:536.
Akinloye O, Arowojolu AO, Shittu OB, Anetor JI. Cadmium toxicity: a possible cause of male infertility in Nigeria. Reprod Biol 2006; 6(1):17–30.
Monsefi M, Alaee S, Moradshahi A, Rohani L. Cadmium‐induced infertility in male mice. Environ Toxicol Chem 2010; 25(1):94–102.
Asadi MH, Zafari F, Sarveazad A, Abbasi M, Safa M, Koruji M, et al. Saffron improves epididymal sperm parameters in rats exposed to cadmium. Nephrourol Mon 2014; 6(1):e12125.
Hosseini B, Nourmohamadi M, Hajipour S, Taghizadeh M, Asemi Z, Keshavarz SA, et al. The effect of omega-3 fatty acids, EPA, and/or DHA on male infertility: A systematic review and meta-analysis. J Diet Suppl 2018; 16:1–2.
Tang LX, Yuan DJ, Wang QL, Jiang F, Guo J, Tang YG, et al. Association of decreased spermatozoa omega-3 fatty acid levels and increased oxidative DNA damage with varicocele in infertile men: a case control study. Reprod Fertil Dev 2016; 28(5):648–54.
Bauer JL, Kuhn K, Al-Safi Z, Harris MA, Eckel RH, Bradford AP, et al. Omega-3 fatty acid supplementation significantly lowers FSH in young normal weight women. Fertil Steril 2017; 108(3):257–8.
Pillai A, Priya L, Gupta S. Effects of combined exposure to lead and cadmium on the hypothalamic–pituitary axis function in proestrous rats. Food Chem Toxicol 2003; 41(3):379–84.
Yang XF, Zhu W, Wei Q, Lin ZN. Effect on apoptosis of anterior pituitary induced by cadmium chloride and its relations with p38 MAPK & ERK1/2 passway. Wei sheng yanjiu. Am J Hyg 2005; 34(6):681–4.
Chouchene L, Pellegrini E, Gueguen MM, Hinfray N, Brion F, Piccini B, et al. Inhibitory effect of cadmium on estrogen signaling in zebrafish brain and protection by zinc. J Appl Toxicol 2016; 36(6):863–71.
Johnson MD, Kenney N, Stoica A, Hilakivi-Clarke L, Singh B, Chepko G, et al. cadmium mimics the in vivo effects of estrogen in the uterus and mammary gland. Nat Med 2003; 9(8):1081.
Sellers RS, Mortan D, Michael B, Roome N, Johnson JK, Yano BL, et al. Society of Toxicologic Pathology position paper: organ weight recommendations for toxicology studies. Toxicol Pathol 2007; 35(5):751–5.
Arafa MH, Mohammad NS, Atteia HH. Fenugreek seed powder mitigates cadmium-induced testicular damage and hepatotoxicity in male rats. Exp Toxicol Pathol 2014; 66(7):293–300.
Aktas C, Kanter M, Erboga M, Ozturk S. Anti-apoptotic effects of curcumin on cadmium-induced apoptosis in rat testes. Toxicol Ind Health 2012; 28(2):122–30.
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