The antibacterial effect of 10% Salvadorapersica (Siwak), 10% green tea, and 50% lemon juice on Enterococcus faecalis: an invitro study

Nsar Muhyaddin Aziz; Venus Dilshad Najeeb

doi:10.15218/zjms.2018.047

Authors

Nsar Muhyaddin Aziz Department of Conservative Dentistry, College of Dentistry, Hawler Medical University, Erbil, Iraq.
Venus Dilshad Najeeb Department of Basic Sciences, College of Dentistry, Hawler Medical University, Erbil, Iraq.

DOI:

https://doi.org/10.15218/zjms.2018.047

Keywords:

Siwak, Green tea, Lemon juice, NaOCl, Enterococcus faecalis

Abstract

Background and objective: Shaping and disinfection of the root canal with a combination of chemical agents and endodontic instruments play an important role in the success of endodontic therapy. This study aimed to evaluate the antibacterial properties of herbal alternatives as possible irrigates against Enterococcus faecalis during endodontic treatment compared with the conventional irrigation solutions such as NaOCl 5%.

Methods: A 10% Siwak extract, 10% green tea extract and 50% fresh lemon juice were prepared separately to make antibiotic disks and seeded on the culture media respectively and compared with 5% NaOCl, distilled water as the control. Plates were left to incubate for 48 hours at 37C^ₒ. Zones of inhibition of the bacterial growth were calculated to measure the antibacterial effect of the tested irrigants.

Results: All irrigants were active against this bacterium. The 5% NaOCl had the highest mean value (27.70±0.66), followed by 10% Siwak (Salvadorapersica) (21.60±0.7), 50% lemon juice (15.10±0.56), and 10% green tea (10.50±0.5).

Conclusion: Herbal extracts can be used as the possible irrigant to compensate 5% NaOCl during endodontic treatment.

Metrics

Metrics Loading ...

References

Al- Azzawi AJ. The antibacterial effect of herbal alternative, green tea and salvadorapersica (siwak) extracts on Enterococcus faecalis. J Bagh 2015; 27(2):1–5.

Paul J. Recent trends in irrigation in endodontics. Int J Microbial 2014; 3(12):941–52.

Jain P, Manish R. Role of herbs in root canal irrigation: A review. JPBS 2014; 9(2):6–10.

Khan R. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strain of bacteria and fungus of clinical origin. Molecules 2009; 14(2):586–97.

Prabhakar J, Senthilkumar M, Priya MS. Evaluation of antimicrobial efficacy of herbal alternatives (Triphala and Green tea poly phenols) MTAD and 5% sodium hypochloride against Enterococcus faecalis biofilm formed on tooth substrate: An invitro study. J Endod 2010; 36:83–6.

Bansode DS, Charan MD. Studies on antimicrobial activity and phytochemical analysis of citrus fruit juice against selected enteric pathogen. IRJD 2012; 3(11):122–6.

Dimic GR, Tanakov SD, Jovanov OO. Antibacterial activity of lemon, caraway and basil extracts on listeria ssp. APTEFF 2012; 43:239–46.

Avwioro G, Lyiola S, Aghoghorwia B. Histological and biochemical markers of the liver wistar rat on subchronic oral administration of green tea. North AMJ Med Sci 2010; 2(8):376–80.

Al-NidawiA. Effect of siwak extracts on mutans streptococci, in comparison to selected antimicrobial agents (in-vitro and in vivo study). M.Sc. Thesis, Department of preventive Dentistry, College of Dentistry, University of Bagdad; 2004.

Maheshwari N. Clinical Microbiology and Parasitology (for DMLT students). 2nd ed. aypee Brothers Medical Publishers; 2008. P. 111–3.

Hulsmann M, Heckendirff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 2003; 36:810–30.

Gomes BP, Ferraz CC, Vianna ME. Invitro antimicrobial activity of several concentrations of sodium hypochloride (NaOCl) and chlorhexidinegluconat in the elimination of Enterococcus faecalis. Int Endod J 2001; 34:424–8.

Berber VB, Gomes BP, Sena NT. Efficacy of various concentration of NaOCl and instrumentation techniques in reducing E. faecalis with in root canals and dentinal tubules. Int Endod J 2006; 39:10–7.

Duntner J, Mines P, Anderson A. Irrigation trends among American Association of Endodontic Members. A web based survey. J Endod 2012; 38(1):37–40.

Ali SS, Kasoju N, Luthra A. Herbs as source of antioxidant. Food Res Int 2008; 41:1–5.

Sudhir K, Devendra S Vijay K. Evaluation the antibacterial activity of plant extracts against bacterial pathogens. JDDT 2012; 2(4):82–5.

Sahni A, Chandak MG. Herbal usage in root canal irrigation: A review. Int J Dent Health Sci 2015; 2(1):76–82.

Al- Sabawi NAK, AL-Sheikhabdal AK, Taha MY.The antimicrobial activity ion of salvadorapersica solution (Miswak- Siwak) as root canal irrigant. University of Sharjah Journal of Pure and Applied Sciences 2007; 4(3):69–91.

Kandaswamy D, Venkateshbabu N, Gogulnath D, Kindo AJ. Dentinal tubule disinfection with 2% chlorexidine gel, propolis, morindacitrifolia juice, 2% povidiniodin and calcium hydroxid. Int Endod J 2010; 43:419–23.