IDENTIFICATION OF ADENOSINE-3', 5'-CYCLIC MONOPHOSPHATE PURUFIED FROM LEUKAEMIC HUMAN URINE
Keywords:CAMP, Human urine
Background: Human cAMP was found to act as a 2nd.messenger responsible for intercellular effects. Its altered levels can exhibit a characteristic response to the metabolic behavior of the cell. Increases in cAMP levels were leads to a variety of responses, dependent on the nature of the target cell. This increases can diffuses to extracellular fluids and can be useful in diagnostic purposes.
Methods: Twenty four hours urine samples of the human leukemia patients (no.86) and normal healthy control were collected, these were includes male and female. 90 samples of healthy controlled were used for comparing data obtained. Levels of cAMP were determined in both groups. Identification of the purified cAMP peaks were achieves using UV, TLC and HPLC analysis.
Results: An elevation in purified cAMP levels were found to be of 15.87 � 2.4 �mole/24 hours and 20.13 � 0.881 �mole/24 hours of both Normal and leukaemic patient�s urine. Identification of the cAMP purified peaks were, reflects the following data: the Rf value of both groups was the same as compared with the standered cAMP used, the retention times of the cAMP peaks were the same, and the UV analysis in different PH values were the same.
Conclusion: Hormones that increase hepatic (glycogen) or renal (parathyroid hormone) cAMP bring about an increased level of the cyclic nucleotide in blood and urine, respectively. Although this may be useful for diagnostic purposes, extracellular cAMP has little or no biologic activity in mammals, several studies have demonstrated that determination of cAMP in 24 hour urine specimens might be useful for the clinical investigation of endocrine disorders, especially of the parathyroid [8-13] and thyroid gland [14,15 ]. The evaluation of leukaemic urinary cAMP found in this study can give an obvious idea about the metabolic behavior of abnormal cell proliferation.
Sutherland E W, Oye I, and Butucher R W, Recent. Prog. Horm. Res. (1965). 12: 623 �646.
Seeley R P; Stephens T D and Tate P, �Essential of Anatomy and Physiology�, 2nd Ed, (1996). McGraw Hill Company USA.
Northrop G and Parks R E, J. Pharmacol. Ther. (1969 a). 145:87.
Hess M E, Hottenstein D, Shanfield J, and Haughard N, J. Pharmacol. Exp. Ther, (1963). 141: 274.
Vaughan M, and Steinberg D, J. Lipid. Res. (1963). 4: 194.
Lundholm L, Lundholm E M, and Svedmyr N, Pharmacol. Rev, (1966) 18:255.
Matin D W, Mayes P A, and Rodwell V W, In: Harper`S Review of Biochemietry, 18 Edition (1981), Pp- 171, 464.
Alston W C, Allen K R, and Tovey J E, Clin. Indocrinol (Oxf). (1980) 13:17. -Broadus A E, Mahaffey J E, Barter F C, and Neer R M, J. Clin. Invest. (1977) 60:771.
Broadus A E, Mahaffey J E, Barter F C, and Neer R M, J. Clin. Invest. (1977) 60:771.
Drezner, M. K., Neelon, F. A., Curtis, H. B., and Lebovitz, H. E. Metabolism, (1976). 25: 1103-1112.
Kaminisky N I, Broadus A E, Hardman J G, Jones Jr D J, Ball J H, Sutherland E W, and Sutherland E W, J. Clin. Invest. (1970) 49:2387.
Neelon F A, Drenzer M, Birch B M, and Lebovitz H E. Lancet, (1973)1:631-634.
Pak C Y C, Adva. Cycl. Nucleo. Res. (1980)12:393.
Gutter R B, Shaw J W, Otis C L, Nicoloff J T, J. Clin. Endocrinol Metab. (1975) 41.
Carter D J and Heath D A, Acta Endocrinol (Copenh) (1977) 84:542.
Dohan P H, Yamashita K, Larsen P R, Davis B, Deftos L, and Field J B. Journal of Clinical Endocrinology and Metabolism, (1972) 35:775-784.
Murad F, and Pak C Y C. New England Journal of Medicine, (1972) 286:1382-1387.
Shaw J W, Oldham S B, Rosoff L, Bethune J E, and Fichman M P. Journal of Clinical Investigation, (1977) 59:14-21.
Mundy G R, Luben R A, Raisz L G, Oppenheim J J, and Buell D N. New England Journal of Medicine, (1974) 290:867-871.
Seyberth H W, Segre G V, Morgan J L, Sweetman B J, Potts J T Jr, and Oates J A. New England Journal of Medicine, (1975) 293:1278-1283.
Scavennec J, Carcassone Y, Gastaut J A, Blanc A, and Cailla H L, Cancer Res. (1981) 41:3222.
Gennari C, Francini G, Galli M, and Lore F, J. Clin. Pathol. (1978) 31:735.
Peracchi M, Lombardi L, Toschi V, Bamonti-Catena F, Mandelli V, Maiolo A T, Baldini L, and Polli E E, Cancer (1984) 54:3028.
Dusheiko G, Levin J, Kew M, Cancer (1981) 47:113.
Brooker G, Method of Biochemical Analysis (1974) 22:95.
Raij K, and Harkonen M, Scand. J. Clin. Lab. Invest. (1976) 36(2): 161.
Chase L R, Melson G L, and Aurbach G D. J. Clin. Invest. (1969) 48: 183
Abdulla Y H, and Hamadah K, Lancet (1970) 21:378.
Mohamad A L, Msc Thesis Mosul University (1991).
Budavari S, the Merk Index, an Encyclopedia of Chemicals, Drugs and Biologicals Eleventh Edition (2003) Published By Merk and Co Inc P-2718.
Newton R P,Chiatante D, Ghosh D, Brown A G, Walton T J, Harris F M, and Brown E G, Phytochemistry (1989) 28(9):2243-2254.
Brooker G, J. Boil. Chem. (1971) 246(24):7810-7816.
Smith C J, Little J P, and Milton M J, Biochem. Soc. Trans. (1989) 17:199-200.
Aurbach G D, Potts J T , Chase L R, and Melson G L. Ann. Intern. Med. (1969) 70: 1243.
Krstulovic A M, Hartwick R A, and Brown P R, Clin. Chem. (1979) 25(2):235-241.
Hassan H G, PhD thesis, university of wales. (1990), p: 3, 10.
Robinson G A, Butcher R W and Sutherland E W, Eds. cAMP Publ. Academic. Press New York and London, (1971). 150-151.
Kuo J F, and Greengard P, Adv. Cyc. Nuc. Res. (1972) 2:41.
Skoog D A, West D M, Holler F J, and Crouch S R, In:� Fundamentals of Analytical Chemistry� Eighth Edition (2004), P-973.
How to Cite
The copyright on any article published in Zanco J Med Sci is retained by the author(s) in agreement with the Creative Commons Attribution Non-Commercial ShareAlike License (CC BY-NC-SA 4.0).