Lipid Peroxidation and Antioxidant Status in Post-Operative Patients with Cancer Treated with Chemotherapy


  • Imad AJ Thanoon Departments of Pharmacology, Surgery, Mosul College of Medicine, University of Mosul
  • Faris A. Ahmed Department of Physiology, Ninevah College of Medicine, University of Mosul, Iraq.
  • Khalaf R. Jadoaa Departments of Pharmacology, Surgery, Mosul College of Medicine, University of Mosul


lipid peroxidation, total antioxidants status, chemotherapy, cancer


Background and Objectives: The aim of this study was to assess the effect of surgical interference and chemotherapy on lipid peroxidation and antioxidant status in patients with cancer disease.

Methods: Twenty one male patients with gastric, colonic, and prostate cancer; and 26 apparently healthy male subjects as a control group, were included in this study. Blood samples (5 ml) were taken from patients and controls and analysed for serum malondialdehyde (MDA) and total antioxidant status (TAS). Blood samples were taken from patients one week before and after the surgery and other blood samples were taken two weeks after the first dose of chemotherapy.

Results: In the cancer patients, serum MDA was significantly higher (P<0.001), while serum TAS was significantly lower (P<0.001) compared with the controls. After surgical removal of cancer, serum MDA was decreased significantly (P<0.001), but serum TAS was increased significantly (P<0.001) compared with the patients before surgery. Chemotherapy treatment further increased serum MDA significantly (P<0.001), with a significant (P<0.001) decrease in serum TAS compared with the measurements after operation.

Conclusions: Surgical removal of cancer decreased lipid peroxidation, and increased antioxidant status. Chemotherapy of cancer increased lipid peroxidation and depressed antioxidant defense system. Oxidative stress might be a consequence and not a cause of cancer.


Metrics Loading ...


- Surapaneni KM, Venkata GR. Lipid peroxidation and antioxidant status in patients with carcinoma of prostate. Indian J Physiol Pharmacol 2006; 50: 350-354.

- Turkdogan MK; Sekeroglu R., Hekim H, Avci E. Lipid peroxidation and upper gastrointestinal cancers. Eastern J Med 1998; 3: 39-42.

- Skrzydlewska E, Sulkowski S, Koda M, Zalewski B, Kanczuga-Koda L, Sulkowska M. Lipid peroxidation and antioxidant status in colorectal cancer. World J Gastroenterol 2005; 11: 403-406.

- Dogru-Abbasoglu S, Aykac-Toker G, Kocak T, Unluer E, Uysal M. Antioxidant enzyme activities and lipid peroxides in the plasma of patients with benign prostatic hyperplasia or prostate cancer are not predictive. J Cancer Res Clin Oncol 1999; 125: 402-404.

- Gerber M, Astre C, Segala C, Saintot M, Scali J, Simony-Lafontaine J, et al. Tumor progression and oxidant-antioxidant status. Cancer lett 1997; 114: 211-214.

- Srivastava DSL, Mittal RD. Free radical injury and antioxidant status in patients with benign prostate hyperplasia and prostate cancer. Indian J Clin Biochem 2005; 20: 162-165.

- Batcioglu K, Mehmet N, Ozturk IC, Yilmaz M, Aydogdu N, Erguvan R, et al. Lipid peroxidation and antioxidant status in stomach cancer. Cancer Invest 2006; 24: 18-21.

- Scibior D, Skrzycki M, Podsiad M, Czeczot H. Glutathione level and glutathione-dependent enzyme activities in blood serum of patients with gastrointestinal tract tumors. Clin Biochem 2008; 41: 852-858.

- Faber M, Coudray C, Hida H, Mousseau M, Favier A. Lipid peroxidation products, and vitamin and trace element status in patients with cancer before and after chemotherapy, including adriamycin. A preliminary study. J Biol Trace Element Res 1995; 47: 117-123.

- Papageorgiou M, Stiakaki E, Dimitriou H, Malliaraki N, Notas G, Castanas E, et al. Cancer chemotherapy reduces plasma total antioxidant capacity in children with malignancies. Leukemia Res 2005; 29: 11-16.

- Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-310.

- Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 1993; 84: 407-412.

- Aydin A, Arsova-sarafinovska Z, Sayal A, Eken A, Erdem O, Erten K, et al. Oxidative stress and antioxidant status in non-metastatic prostate cancer and benign prostatic hyperplasia. Clin Biochem 2006; 39: 176-179.

- Skrzycki M, Majewska M, Podsiad M, Czeczol H. Expression and activity of superoxide dismutase isoenzymes in colorectal cancer. Acta Biochem Pol 2009; Epub ahead of print.

- Bandyopadhyay U, Das D, Banerjee RK. Reactive oxygen species: oxidative damage and pathogenesis. Current sci 1999; 77: 658-666.

- Comstock GW, Alberg AJ, Huang HY, Wu K, Burke AE, Hoffman SC, et al. The risk of developing lung cancer associated with antioxidants in the blood: ascorbic acid, carotenoids, α-tochpherol, selenium and total peroxyl radical absorbing capacity. Cancer Epidemiol Biomarker Prev 1997; 6: 907-916.

- Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 2005; 15: 316-328.

- Czeczot H, Scibior D, Skrzycki M, Podsiad M. Antioxidant barrier in patients with gastric cancer-preliminary study. Pol Merkur Lekarski 2005; 19: 521-525.

- Skrzydlewska E, Stankiewicz A, Sulkowska M, Sulkowski S, Kasacka I. Antioxidant status and Lipid peroxidation in colorectal cancer. J Toxicol Environ Health A 2001; 64: 213-222.

- Look MP, Musch E. Lipid peroxides in the polychemotherapy of cancer patients. Chemotherapy 1994; 40: 8-15.

- Conklin KA. Cancer chemotherapy of antioxidants. J Nutr 2004; 134: 3201S-3204S.




How to Cite

Thanoon, I. A., Ahmed, F. A., & Jadoaa, K. R. (2018). Lipid Peroxidation and Antioxidant Status in Post-Operative Patients with Cancer Treated with Chemotherapy. Zanco Journal of Medical Sciences (Zanco J Med Sci), 14(3 Special), 64–67. Retrieved from



Original Articles