Inhibitory effect of gundelia extract on urinary α-amylase activity of type-I diabetes mellitus

  • Namam S. Hamad Dept. Chemistry, College of Science, Sulaimani Univ., Sulaimania – IRAQ.
  • Hamid G. Hasan Dept. Chemistry Ibn Al-Haitham College of Education,Univ. of Baghdad
Keywords: α-Amylase, Gundelia extract,, Diabetes mellitus-I-.


Background: α-amylase is an enzyme that degrades starch into maltose and glucose by hydrolyzing α-1,4-glucan bonds.It is known that the enzyme is found to be elevated in patients suffering from diabetes mellitus.A potent extracts of locally distributed wild plant,the Gundelia was found to inhibit elevated activity of α-amylase with dose responses.

Methods: Samples of 50 diabetes mellitus type-I has been investigated for urinary α-amylase activity.An inhibitory dose of Gundelia extract were used for the inhibition of the elevated enzyme activity.The data obtained were compared with that of healthy controlled samples﴾30﴿.The adopted protocol was a colorimetric determination﴾Anonymous,1980﴿ .

Results: α-amylase activity was found to be elevated﴾281.70±10.03 IU/24hr) in patient’s serum compared with that of controlled samples﴾43.38±3.33 IU/24hr﴿.The activity of the enzyme was found to be inhibited﴾160.11±250.4 IU/hr﴿ in patients serum using dose response of 15mg/ml of Gundelia extract. The study were comprehensive to determined the physical parameters of the enzyme activity and a values of Vmax and Km were obtained.

Conclusion: It was known that Gundelia is used in prevention and treatment of liver diseases. The plant has a role in the body as an antioxidant factor.It has a hypolipemic effect, therefore, a use of such plant extract could have a hypoglycemic activities on patients with DM-I.


- Anonymous.1980. Procedure 700"Amylase: Visual, Colorimetric determination in serum or urine "Sigma chemical company, Sigma Diagnostics.

- Geerling G, Honnicke K,et al. Graefes Arch clin Exp Ophthalmol.2000,238:45- 52.

- Searcy, R. L, Wilding, p, et al. clin chim acta 1967, 15,189.

- Henry, R.L. and Chiamori,N. clin chem. 1960,5:434

- Young, D.S, Pestaner, L.C. et al clin chem. 1975, 21:10

- Yadav, D. Nair S. et al. Am J Gastro entrol .2000, 95:3123- 3128

- Nair S.Yadav, Pitchumoni CS. Am J Gastro entrol. 2000, 95:2795-2800

- Ali A. Rizivi, MD. Diabetes Care .2003, 26: 3193-3194

- H. Fluk. Medicinal plant and their uses. 4th edition, London (1977)

- Karis, P.O, Eldenas, et al. Taxon, 2001, 50:105-114, Issn 0040-0262.

- AAcc., 1987 Approved methods o the AAcc, American Associated of coreal chemists INC, stpan, Minn.

- Akram Jamshidzadeh, Fatema Fereioni, et al. J of Ethno pharmacology 2005,101, Issues 1-3, pp-233-237

- Coruh, N; Sagdcoglucelep, AG, et al, Food Chemistry 2007, 100, no-3, pp.1249- 1253

- Sharaf, KH; Ali,JS. Veterinnarski Archiv, 2004, 74, no-5, pp-359-370

- Henricksson, J, et al. J. Cardio Risk.1995, 2(4): 303-309

- Tsuyshi Yamada, Ryuichi Moriyama, et al. Plant science.2005, 169(3), pp- 502-511

- Hamadan and F.U.Afifi. J. of Ethanopharmacology.2004, 93(1), pp.117-121

How to Cite
Hamad, N., & Hasan, H. (2018). Inhibitory effect of gundelia extract on urinary α-amylase activity of type-I diabetes mellitus. Zanco Journal of Medical Sciences (Zanco J Med Sci), 14(3), 1 - 6. Retrieved from
Original Articles