Effect of cigarette smoking on liver function test and some other related parameters


  • Sangar Najat Abdul-Razaq Health Ministry, Health Office, Kirkuk/Iraq
  • Bakhtiar M. Ahmed Department of Basic Science, College of Dentistry, Hawler Medical University, Erbil, Iraq




Cigarette smoking, Liver function test, Liver enzymes, MAD, Lipid profile


Background and objective: Cigarette smoking is one of the 10 greatest contributors to global death and disease. Cigarette smoke consists of many chemicals, including cytotoxic, carcinogenic and free radicals, therefore it affects many organs if not all.This work is directed to evaluate the effects of dose response patterns of tobacco exposure on liver tissue, through assessing some serum biochemical parameters related with liver  efficiency.

Methods: This study was done in Kirkuk province. It was conducted on (110) healthy male subjects, their ages ranged from 20 to 40 years. They were divided into three groups; 40 heavy smokers, 30 moderate smokers and 40 non-smokers. Blood was withdrawn for estimation of serum liver function test, lipid profile, protein electrophoresis, and lipid peroxidation test (Serum Malondialdehyde; MAD level).

Results: There were statistically significant elevations in serum alkaline phosphatase (ALP), alanine transaminase(GPT) and aspartate aminotransferase(GOT) activities in heavy smokers while serum total bilirubin significantly was lower comparing to non smokers. Serum total protein and albumin were significantly lower in heavy smokers comparing to non smokers. The results of serum protein agarose gel-electrohporesis showed significant changes in serum protein fractions in smoker groups. The mean level of serum total cholesterol, triglyceride, LDL, VLDL and malondialdehyde was significantly higher in heavy smoker group, while serum HDL level had a significantly lower value.

Conclusion: Cigarette smoking can affect liver efficiency and functions. These effects are dose exposure depenent.


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Funck C., Mathilde R., Michel L., Arnould J., Verstuyft C., Martine L., et al.. Effects of type of smoking (pipe, cigars or cigarettes) on biological indices of tobacco exposure and toxicity. Lung Cancer. 2006;54: 11–8.

Aurelio, L.. Biochemical markers of cardiovascular damage from tobacco smoke. Curr. Pharm. Des. 2005;11: 2190–208.

Janson C., Chinn S., Jarvis D., Zock J., Torén K. and Burney P. Effect of passive smoking on respiratory symptoms, bronchial responsiveness, lung function, and total serum IgE in the European Community Respiratory Health Survey: a cross-sectional study. Lancet. 2001;358 (9299):2103-9.

Lubin J.H., Alavanja M.C., Caporaso N, Brown L.M., Brownson R.C., Field R.W., et al. Cigarette smoking and cancer risk: modeling total exposure and intensity. Am J Epidemiol. 2007;166(4): 479-89.

Yeh, C., Graham B, Powell C., Mesia-Vela S., Wang Y., Hamade N., et al. Effect of cigarette smoking dose on oxidized plasma proteins. Environ. Res.2008; 106: 219–25.

Yuen S.T., Gogo A.R., Luk I.S., Cho C.H., Ho J.C. and Loh T.T. The effect of nicotine and its interaction with carbon tetrachloride in the rat liver. Pharmacol. Toxicol.1995; 77: 225–30.

Benowitz N., Hall S., Stewart S., Wilson M., Dempsey D. and Jacob P. Nicotine and Carcinogen Exposure with Smoking of Progressively Reduced Nicotine Content Cigarette. Cancer Epidemiol. Biomarkers Prev. 2007; 16: 2479–85.

David E.E., Shashank J.D. and Hongsheng G. Effect of cigarette Smoking on pentane excretion in alveolar breath. Clinical chemistry 1996; 42(2): 103-308.

Spiro S. G. and Silvestri G. A. (2005). One hundred years of lung cancer. Am. J. Respir. Crit. Care Med.; 172: 523–9.

Yu M.W., Hsu F.C., Sheen I.S., Chu C.M., Lin D.Y., Chen C.J., et al. Prospective study of hepatocellular carcinoma and liver cirrhosis in asymptomatic chronic hepatitis B virus carriers. Am J Epidemiol. 1997; 145: 1039-47.

Pessione F., Ramond M.J., Njapoum C., Duchatelle V., Degott C., Erlinger S., et al. Cigarette smoking and hepatic lesions in patients with chronic hepatitis C. Hepatology. 2001; 34: 121-5.

Martin A and Crook T. Normal female gonadal function in: Clinical chemistry and metabolic medicine, 7thed hodder Arnold. 338Eeuston road, London. 2006:148-9.

King P.R. and King E. J. Estimation of plasma Phosphatase by determination of hydrolysed phenol with amino-anti-antipyrine. J. Clin. Path.1954; 7:322-326.

Frankl H.D. and Merrit J.H. Enzyme activity in the serum and common bile duct. Am J Gastroenterol. 1959; 31: 166-9.

Walters M. and Gerarde H. Colourmeteic evalution of serum bilirubin. Microchem J. 1970; 15:231-243.

Tietz N.W. Text book of clinical chemistry, 3rd Ed. C.A. Burtis, E.R. Ashwood, W.B. Saunders 1999.

Doumas B.T. and Biggs H.G. Determination of serum albumin,– Standard methods of clinical chemistry – Acad. Press. N.Y.1972; 7:175-188.

Clarke W. and Dufour D.R. Contemporary Practice in Clinical Chemistry. AACC Press; Washington. 2006: 200-206.

Hames B. D. Gel Electrophoresis of Proteins: A Practical Approach 3rd ed., Oxford University Press, New York. 1998:341-55.

Botsoglou, N.A., Rapid, Sensitive, and Specific Thiobarbituric Acid Method for Measuring Lipid Peroxidation in animal Tissue, Food and Freestuff Samples, J. Agric. Food Chem. 1994; 42:1931-7.

Jentzsch, A.M., et al., Improved Analysis of Malondialdehyde in Human Body Fluids, Free Rad. Biol. Med. 1996; 20:251-6.

Chakraborty A. and Selvaraj S. Differential modulation of xenobiotic metabolizing enzymes by vanadium during diethylnitrosamine induced hepatocarcinogenesis in Sprague-dawley rats. Neoplasma. 2000; 47: 81– 9.

Pannuru P., Vaddi D. and Nallanchakravarthula V. Influence of Chronic Cigarette Smoking on Serum Biochemical Profile in Male Human Volunteers. Journal of Health Science. 2009; 55(2) 265–70.

Halliwell B. Albumin – an important extracellular antioxidant. Biochem Pharmacol. 1988; 37: 569-71.

Hu M., Louie S., Cross C., Motchnik P. and Halliwell B. Antioxidant protection against hypochlorous acid in human plasma. J Lab Clin Med. 1993; 121: 257-62.

Wilhelmsen L. Coronary heart disease. Epidemiology of smoking & intervention studies of smoking. Am Heart J. 1988; 115: 242-247.

Gill H.C. The cardiovascular pathology of smoking. Am Heart J. 1988; 115(1 Pt 2): 250– 7.

Neki N.S. Lipid Profile in Chronic Smokers – A Clinical Study. JIACM. 2002; 3(1): 51- 4.

Simons L.A., Simons J. and Jones A.S. The interaction of body weight, age, cigarette smoking and hormone usage with blood pressure and plasma lipids in an Australian community. Aus NZ J Med. 1984; 14: 215-21.

Frei B., Forte T.M., Ames B.N. and Cross C.E. Gas phase oxidants of cigarette smoke induce lipid peroxidation and changes in lipoportein properties in human blood plasma. Biochem J. 1991;277:133- 8.

Pryor W. and Stone K. Oxidants in cigarette smoke. Radicals, hydrogen peroxide, peroxynitrate and peroxynitrite. Ann. N. Y. Acad. Sci.1993; 686: 12–27.

Lykkesfeldt J., Viscovich M. and Poulsen H. Ascorbic acid recycling in human erythrocytes is induced by smoking in vivo. Free Radic Biol Med. 2003; 35: 1439– 47.

Agarwal, R. Smoking, oxidative stress and inflammation: impact on resting energy expenditure in diabetic nephropathy. BMC Nephrol. 2005; 6: 13–21.

Jaimes E., Tian R. and Raij L. Nicotine: The Link Between Cigarette Smoking and the Progression of Renal Injury. Am. J. Physiol. Heart Circ. Physiol. 2007; 292: 76–82.




How to Cite

Abdul-Razaq, S. N., & Ahmed, B. M. (2013). Effect of cigarette smoking on liver function test and some other related parameters. Zanco Journal of Medical Sciences (Zanco J Med Sci), 17(3), 556–562. https://doi.org/10.15218/zjms.2013.0048



Original Articles