HPLC analysis and antidiabetic effect of Rheum ribes root in type 2 diabetic patients
Background and objective: Rheum ribes (polygonaceae) roots are used traditionally to treat diabetes, hypertension, obesity, ulcer, diarrhea, anthelmintic and expectorant. The purpose of this study was to investigate clinically the antidiabetic activity of Rheum ribes in type 2 diabetes mellitus patients and phytochemical study for the correlation of antidiabetic activity with the active constituents in the plant.
Methods: The antidiabetic activity of Rheumribes given alone and in combination with standard oral hypoglycemic agents was investigated in type 2 diabetes mellitus. The study was conducted on 120 patientswith type 2 diabetes mellitus for a period of 12 weeks. Preliminary phytochemical screening was carried out followed by high performance liquid chromatography analysis for the identification of flavonoid constituents in the root of Rheumribes.
Results: Rheumribes root showed significant blood glucose reduction (P <0.01) on 12th weeks of the observation period with 39.63% percent blood glucose reduction. The combination treatment of Rheumribes root and glibenclamide showed a significant difference (P <0.05) with the treatment group of glibenclamide and metforminwith maximum percent of reduction in blood glucose level of 48.91%.The results of preliminary phytochemical screening showed the presence of alkaloids, tannins, flavonoids, anthraquinones, and quinones. Quercetin was identified by high performance liquid chromatography. The proposed high performance liquid chromatography method was validated for linearity, accuracy, precision and limit of quantitation.
Conclusion: Rheum ribes root was found to reduce significantly blood glucose levels in type 2 diabetes mellitus patients.Quercetin was identified by high performance liquid chromatography as an important flavonoid constituent.
Nathan DM, Buse JB, Davidson MB. American Diabetes Association, European Association for Study of Diabetes. Medical management of hyperglycemia in type 2 diabetes: a consensus algorithm for the initiation and adjustment of therapy: a consensus statement of the American Diabetes Association and the European Association for the Study of Diabetes. Diabetes Care 2009;32: 193-203.
Whiting DR, Guariguata L, Weil C, Shaw J. IDF Diabetes Atlas: Global estimates of the prevalence of diabetes for 2011 and 2030. Diab Res ClinPract2011;94: 311-21.
Van Dam RM, Willett WC, Rimm EB, Stampfer MJ, Hu FB. Dietary fat and meat intake in relation to risk of type 2 diabetes in men. Diabetes Care 2002;25:417-24.
Acosta-Patino JL, Jimenez-Balderas E, Juarez-Oropeza MA, Diaz-Zagoya JC. Hypoglycemic action of Cucurbitaficifolia on type 2 diabetic patients with moderately high blood glucose levels. J Ethanopharmacol 2001;77: 99-101.
Sharifi S, Asrar D. Rhubarb. Hafez-e- Novin press. Iran 2005;539-42.
Zargari A.Rhubarbes S.Medicinal plant. 6th ed. Tehran University publication 1997;4(6):234-9.
Abu-Irmaileh BE, Afifi FU. Herbal medicine in Jordan with special emphasis on commonly used herbs. J Ethanopharmacol 2003;89:193-7.
Tabata M, Sezik E, Honda G, Yesilada E, FukiH,Goto K. Traditional medicine in Turkey III. Folk Medicine in East Anatolia, Van and Bitlis provinces. Int J Pharmacogn1994;32:3-12.
Alaadin AM, Josefsen K., Pedersen ME, Jäge AK. Hypoglycemic activity of Iraqi Rheum ribes root extract. Pharm Biol2009; 47(5):380–3.
Ozbek H, Ceylan E, Kara M, Özgökçe F, Koyuncu M. Hypoglycemic effect of Rheum ribes roots in alloxan induced diabetic and normal mice. Scand J Lab AnimSci2004; 2(31):113-5.
Snyder L, Kirkland J, Glajch J. Practical HPLC method development. A wileyIntersci pub1997.
Alaadin M. Naqishbandi, Anna K. Jäger, and Ekbal H. Al-Khateeb. A comparative qualitative and quantitative study of anthraquinone derivatives in the roots of Rheum ribes and Rheum emodi by HPLC. Iraqi J Pharm Sci 2009; 18:60-4.
Barnes J, Anderson LA, Phillipson JD. Herbal Medicines. Pharma Press, London;2007.
Knapp RG, Miller MC. Clinical Epidemiology and Biostatistics. Harwal Pub Co, Malverin, Pennsylvania; 1992; 275-92
Alupuli A, Calinescu I, Lavric V. Ultrasonic vs. microwave extraction intensification of active principles from medicinal plants. AIDIC conference series;2009.
Siddiqui AA, Ali M. Practical Pharmaceutical chemistry. New Delhi1997; 126-31.
Sofowora A. Medicinal plant and traditional medicine in Africa. Screening plant for bioactive agents1993;134-56.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction. IntPharmaSci2011;(1):98-106.
Trease GE, Evans WC. Pharmacognosy. BailliereTindall, London 1989, 45-50.
International Conference on Harmonization, guideline Q2A, Text on Validation of AnalyticalProcedures: Federal Register, Rockville, MD, USA, 1995; 60(40): 11260–2.
International Conference on Harmonization, guideline Q2B, Validation of Analytical Procedures: Methodology, Federal Register, Rockville, MD, USA, 1997; 62(96): 27463–67.
Hui H, Tang G, Liang V. Hypoglycemic herbs and their action mechanisms. Chin Med2009;4:11-4.
Fallah HH, Fakhr ZH, Larijani B, Sheikh SA. Review of anti-diabetic medicinal plants used in traditional medicine. J of Med Plants 2006; 5:60-85.
Alaadin AM, Al-Khateeb EH, Jäger AK. Antibacterial activity of the Iraqi Rheumribes root. PharmaBiol2007;45:688–90.
Baytop T. Therapy with Medicinal Plants in Turkey. Nobel Tıp Kitabevleri: Istanbul;1999; 319-20.
Octay M, Yildirim A, Bilaloglu V, Gulcin I. Antioxidant activity ofdifferent parts of isginRheum ribes L. Asian J Chem2007; 19 (4): 3047-55.
Gholamhoseinian A, Moradi M, Sharifi-far F, AbbasiOshaghi E. Identification of anticholinesterase compound from Berbers integrima, Rheum ribes and Levisticumofficinale. ResPharmaSci2012; 7(5):754.
Aguirre L, Arias N, Macarulla MT, Gracia A and Portillo MP.Beneficial Effects of Quercetin on Obesity and Diabetes. Open Nutraceuticals J2011; 4:189-98.
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