Effect of non surgical periodontal treatment on TNF-α levels in serum of patients with chronic periodontitis

  • Sazan Sartip Abdulhameed Department of Periodontology, college of dentistry, Hawler Medical University, Erbil, Iraq.
  • Haween T. Nanakaly Department of basic sciences, college of dentistry, Hawler Medical University, Erbil, Iraq.
Keywords: Chronic periodontitis, Tumor necrosis factor-α, Chlorhexidine mouth wash, Non-surgical periodontal treatment

Abstract

Background and objective: Chronic periodontitis is a multifactorial inflammatory disease characterized by destruction of tooth supporting tissues. Tumor necrosis factor-α, a key inflammatory cytokine, plays an important role in periodontal destruction. This study aimed to analyze the effect of non-surgical periodontal treatment on tumor necrosis factor-α levels in serum of patient with chronic periodontitis (with and without using chlorhexidine 0.2% mouthwash). Methods: A total of 44 patients with moderate to severe localized chronic periodontitis were divided into: Group I (n=22) without using of chlorhexidine mouthwash and Group II (n=22) with 0.2% chlorhexidine mouthwash. Serum samples collected from each subjects at baseline and four weeks after scaling and root planning treatment were quantified for tumor necrosis factor-α levels using ELISA technique. Results: The mean concentration of TNF-α were significantly reduced one month after treatment in sera of patients with chronic periodontitis for group I when compared to its mean concentration before treatment (P <0.05), while in group II a highly significant reduction after one month treatment (P <0.001) was observed. Conclusion: The effect of non-surgical periodontal therapy with chlorohexidine mouth wash showed more reduction in the levels of serum tumor necrosis factor-α.

References

Mullally BH, Coulter WA, Hutchinson JD, Clarke HA. Current oral contraceptive status and periodontitis in young adults. J Periodo 2007; 78:1031-6.

AbbasAk, lichtman AH.Cellular and molecular immunology. 5th ed, Saunders, The Curtis Center, Independent Square West, Philadelphia, PA; 2005. P.243-75.

Newman MG, Takei HH, Klokkevold PR, Carranza FA. Caranza’s clinical periodontology. In: Chronic periodontitis, 11th ed. St. Louis, MO: Elsevier Saunder; 2012. P.483-6.

Mehmet Sa, Glam U, Gur A, Sxerife BB, Sema SH. Boric acid irrigation as an adjunct to mechanical periodontal therapy in patients with chronic periodontitis: A randomized clinical trial. J Periodontol 2013; 84(9):1297-308.

Sharma S, Saimbi CS, Koirala B,Shukla R.Effect of various mouthwashes on the levels of interleukin-2 and interferon gamma in chronic gingivitis. J Clinpediatr Dent Winter 2008; 32(2):111-4.

Okada H, Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med 1998; 9(3):248-66.

Birkedal H. Role of cytokines and inflammatory mediators in tissue destruction. J Periodontal Res 1993; 28:500-10.

Dufour A, Baran C, Langkamp HL, Piesco NP, Agarwal S. Regulation of differentiation of gingival fibroblast and periodontal ligamentcells by rhIL-1 b and rhTNF-a. J Periodontal Res 1993; 28:566-8.

Manolagas SC.Role of cytokines in bone resorption. Bone 1995; 17:63S-7S.

Kantarci A,Hasturk H, Van Dyke T. Host-mediated resolution of inflammation in periodontal diseases. Periodontol 2006; 40: 144-63.

Lindhe J, Niklaus PL, Karring T. Clinical periodontology and implant dentistry. 5th. Blackwell Munksgard; 2008.

American Academy of Periodontology. Ad Hoc Committee on the Parameters of Care: Phase I therapy. J Perio 2000; 71: 856.

Sood R. Textbook of Medical Laboratory Technology. Chapter 9 ClinicalHaematology 1st ed. New Delhi: Jaypee Brothers Medical Publishers; 2006.

George, AK, Janam P. Theshort-term effects of non-surgicalperiodontal therapy on the circulating levels of interleukin-6 and C-reactive protein in patients with chronic periodontitis. J Indian SocPeriodontol 2013; 17(1): 36-41

Radafshar G, Shad B, Ariamajd E, GeranmayehS. Effective of non-surgical treatment on the level of serum inflammatory markers in advanced periodontitis.J Dent 2010; 7(1): 24-30.

Tuter G, Ozdemar B, Kurtis B, Serdar M, Yucel AA, Ayhan E. Short term effect of non-surgical treatment on gingival crevicular fluid levels of tissueplasminogen activator and plasminogen activator inhibitor 2 in patients with chronic and aggressive periodontitis. Arch oral Biol 2013; 58:391-6.

Radafshar G,Torabi F, Mirfarhadi N. Short-term effects of intensive non-surgical periodontal therapy and low-dose doxycycline on serum levels of IL-6, TNF-α and lipid profile in advanced periodontitis. Afr JMicrobiol Res 2012; 6(2): 355-60.

Chauhan AS, Bains VK, Gupta V, Singh GP, Patil SS. Comparative analysis of hyaluronan gel and xanthan chlorhexidine gel, as adjunct to scaling and root planning with scaling and root planning alone in the treatment of chronic periodontits. ContempClin Dent2013; 4(1):54-61.

Duss C, Lang N P, Cosyn J,Persson G R. A randomized, controlled clinical trial on the clinical, microbiological, and staining effects of novel 0.05% chlorohexidine/herbal extract and a 0.1% chlorohexidinemouthrinse adjunct to periodontal surgery. J ClinPeriodontol 2010; 37(11):988-97.

Matesanz-Pérez P, García-Gargallo M, Figuero E, Bascones-MartínezA, Sanz M, Herrera D. A systematic review on the effects of local antimicrobials as adjuncts to subgingival debridement, compared with subgingival debridement alone, in the treatment of chronic periodontitis. J ClinPeriodontol 2013; 40 (3):227-41.

Lang NP, Schatzle MA,Loe H. Gingivitis as a risk factor in periodontal disease. J Clin Periodontol 2009; 36:3-8.

Caton JC, Zander HA.The attachment between tooth and gingival tissues after periodic root planing and soft tissue curettage. J Periodontol 1979; 50:462.

Dag A, Firat ET, Arikan S, Kardiroglu AK, Kaplan A. The effect of periodontal therapy on serum TNF-α and HbA1c levels in type 2 diabetic patients. Aust Dent J 2009; 54:17-22.

Iwamoto Y, Nishimura F, Nakagawa M, Sugimoto H, Shikata K, Makino H, et al. The effect of antimicrobial periodontal treatment on circulating TNF- α and glycated hemoglobin level in patients with type 2 diabetes. J Periodontol 2001; 72:774-8.

D’Aiuto F, Nibali L, Parkar M, Suvan J, TonettiMS. Short-term Effects of Intensive Periodontal Therapy on Serum Inflammatory Markers and Cholesterol. J Dent Res 2005; 84: 269-73.

Salvi GE, Kandylaki M, TroendleA, Persson GR, Lang NP. Experimental gingivitis in type 1 diabetics: a controlled clinical and microbiological study. J Clin Periodontol 2005; 32:310-6.

Geerts SO, Nys M, De MP, Charpentier J, Albert A, Legrand V, et al.. Systemic release of endotoxins induced by gentle mastication: association with periodontitis severity. J Periodontol 2002; 73:73-8.

Van Deventer SJ, Hart M, Van der Poll T, Hack CE, Aarden LA. Endotoxin and tumor necrosis factor-alpha induced interleukin-8 release in humans. J Infect Dis 1993; 167:454-61

Ide M, Jaqdev D, Coward PY, Crook M, Barclay GR, Wilson RF. The short term effect of treatment of chronic periodontitis on circulating levels of endotoxins,CRP, IL-6 and TNF- α. J Periodontol 2004; 75(3):420-8.

Lundin M, Yucel-Lindberg T, Dahllöf G, Marcus C,Modeer T. Correlation between TNFalpha in gingival crevicular fluid and body mass index in obese subjects. Acta Odontol Scand 2004; 62:273-7.

Yamazaki K, Honda T, Oda T, Ueki-Maruyama K, Nakajima T, Yoshie H, et al. Effect of periodontal treatment on the C-reactive protein andproinflammatory cytokine levels in Japanese periodontitis patients. J Periodontal Res 2005; 40:53-8.

Published
2016-04-10
How to Cite
Abdulhameed, S., & Nanakaly, H. (2016). Effect of non surgical periodontal treatment on TNF-α levels in serum of patients with chronic periodontitis. Zanco Journal of Medical Sciences (Zanco J Med Sci), 20(1), 1167_1174. https://doi.org/10.15218/zjms.2016.0005
Section
Original Articles