Open-label uncontrolled pilot study on antipsoriatic activity of Rosa hemisphaerica


  • Aveen N. Adham Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
  • Lana Y. Muttalib Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
  • Alaadin M. Naqishbandi Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
  • Salah Abu Baker Ali Department of Pathology, College of Medicine, Hawler Medical University, Erbil, Iraq



Rosa hemisphearica, Antipsoriatic, HPLC, TLC, Total phenolic


Background and objective: Rosa hemisphearica (Rosaceae) is highly cultivated worldwide as an ornamental plant and medicinally have an antibacterial and antioxidant effect. This study aimed to investigate clinically and histopathologically the anti-inflammatory activity of Rosa hemisphearica in psoriasis and to correlate the anti-inflammatory activity with the active constituents in the plant by the phytochemical study.

Methods: In this open-label uncontrolled pilot study, a topical preparation of R. hemisphearica stem extract was used by 20 patients suffering from psoriasis for three weeks. Three histopathological slides for each patient, one each week for three weeks was done for anti-inflammatory response evaluation. Subsequently, total phenolic content was measured followed by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis for the identification of phenolic acid content in the stem.

Results: By histopathological examination revealed that both Munro’s and Kogoj’s abscesses were gradually disappeared, the granular layer gradually restored to normal and the inflammatory cells gradually reduced in number prominently polymorph (neutrophils) and eventually histocytes disappeared. Total phenolic contents in R. hemisphearica stem extract were determined as 205.625 µg gallic acid equivalent\ each gm of the sample extract, TLC, and HPLC results showed the presence of rosmarinic acid in the stem extract. On quantitative HPLC analysis, the percentage of rosmarinic acid in the stem extract was 0.024 with a retention time (Rt) of 3.4 min. The proposed HPLC method was found to be linear, accurate and precise.

Conclusion: The organic solvent extract of R. hemisphearica stem showed a significant reduction in an inflammatory cell among psoriatic patients under study. Rosmarinic acid was identified by TLC and HPLC as important phenolic acid constituents.


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Rechinger HK. Flora Iranica. Rosaceae II: Rosa, J. Zielinski. Graz: Akademische Druck und Verlagsanstalt publishing; 1982. p.8.

Zargari A. Medicinal plants, 2nd ed. Tehran: Tehran University Publication; 1991. p.162–4.

Kashani AD, Rasooli I, Sharafi SM, Rezaee MB, Nadoushan MRJ, Owlia P. Phytobiological characteristics of Rosa hemisphaerica Herrm. Extract. J Med Plant 2010; 9(6):97–106.

Basim E, Basim H. Antibacterial activity of Rosa damascena essential oil. Fitoterapia 2003; 74:394–6.

Boskabady MH, Kiani S, Rakhshandah H. Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol 1995; 106:377–82.

Orhan DD, Hartevio˘glu A, K¨upeli E, Yesilada E. In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits. J Ethnopharmacol 2007; 112:394–400.

Safaei-Ghomi J, Bamoniri A, Hatami A, Batooli H. Determination of volatile components in Iranian Rosa hemisphaerica. Chem Nat Comp 2007; 43(6):738–40.

Gudjonsson JE, Elder JT. Psoriasis: epidemiology. Clin Dermatol 2007; 25(6):535–46.

Alupuli A, Calinescu I,Lavric V. Ultrasonic vs. microwave extraction intensification of active principles from medicinal plants. AIDIC conference series; 2009.

Ramanauskienė K, Žilius M, Briedis V. Rheological and biopharmaceutical studies of the experimental propolis semisolid preparations. konferencijosmedžiaga 2012; 18(2):181–8.

Arain M, Campbe JM, Cooper LC, Lancaster AG. What is a pilot or feasibility study? A review of current practice and editorial policy. BMC Med Res Method 2010; 10(67):1–7.

Mcdonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive oil extracts. Food Chem 2001; 73:73–84.

Adesegun SA, Fajana A, Orabueze CI, Coker HAB. Evaluation of antioxidant properties phaulopsis fascisepala. Evid Based Complement Alternat Med 2009; 6:227–31.

Khoddami A, Wilkes AM and Roberts HT. Techniques for analysis of plant phenolic compounds. Molecules 2013; 18:2328–75.

Pavel M, Voştinaru O, Mogoşanu C, Ghibu S. Phytochemical and pharmacological research on some extracts obtained from Serpylli herba. Farmacia 2011; 59(1):77–83.

Nour V, Trandafir I,Cosmulescu S. HPLC Determination of phenolic acids, flavonoids, and juglone in walnut leaves. J Chrom Sci 2012; 6:1–8.

International Conference on Harmonization, guideline Q2A, Text on validation of analytical procedures: Methodology. USA: Federal register publishing; 1995. p. 11260–2.

International Conference on Harmonization, guideline Q2B, validation of analytical procedures: Methodology. USA: Federal register publishing; 1997. p. 27463–7.

Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010; 2:1231–46.

Fadel O, El Kirat K, Morandat S. Anti-inflammatory actions of flavonoids and structural requirements for new design. Biochimica Biophysica Acta 2011; 1808:2973–80.

Theoharides TC, Alexandrakis M, Kempuraj D, Lytinas M. The antitumor activities of flavonoids. Int Immunopathology Pharmacol 2001; 14(3); 119–27.

Kandaswami C, Lee LT, Lee PP, Hwang JJ, Ke Fc, Huang T, et al. The antitumor activities of flavonoids. In vivo 2005; 19(5):895–909.

Vinatoru M. An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason Sonochem 2001; 8:303–13.




How to Cite

Adham, A. N., Muttalib, L. Y., Naqishbandi, A. M., & Ali, S. A. B. (2017). Open-label uncontrolled pilot study on antipsoriatic activity of Rosa hemisphaerica. Zanco Journal of Medical Sciences (Zanco J Med Sci), 21(1), 1636–1644.



Original Articles