Isolation and identification of microorganisms in acne patients


  • Niyan Innam Muhammed Department of Microbiology, College of Medicine, Hawler Medical University, Erbil, Iraq.
  • Rassool A. Dabbagh Department of Microbiology, College of Medicine, Hawler Medical University, Erbil, Iraq.



Acne, Skin flora, Vitek 2 compact system.


Background and objective: Acne is a follicular skin disease most frequently occurring during the adolescent or the teenage years of a person's life. It has been found that around 85% of teenagers suffer from this skin disorder worldwide. However, acne is not only restricted to teenagers but in many cases continues well into adulthood. This study aimed to examine the prevalence of acne in relation to age in Erbil City, and isolation and identification of microorganisms in acne patients. 

Methods: Specimens were collected from inflamed and discharging pus from 160 acne patients (74 male, 86 female) attending the Department of Dermatology in Rizgari Teaching Hospital in Erbil city. The cultures were examined using the Vitek-2 compact system.

Results: Among 160 samples taken, 150 (99%) were culture positive. From there 70 (46.6%) were male and 80 (53.3%) were female. Only ten (6.25%) samples revealed no growth. The highest prevalence of acne was 32 (20.0%) at age group (15-17) years of both genders. The most frequent etiological agent isolated (aerobically) bacteria from acne patients was Staphylococcus epidermidis (27.08%), and the most common anaerobic pathogen bacteria species were Propionibacterium acnes, 25 (13.02%), while among fungi Candida albicans was the most common fungi representing nine (64.29%) isolates.

Conclusion: Gram-positive bacteria were the most common microorganisms involved in acne infection. There are variations in the incidence of acne infection in relation to sex, age, and socioeconomic level.


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Adityan B, Kumari R, Thappa DM. Scoring systems in acne vulgaris. Indian J Dermatol Venereol Leprol 2009; 75(3):323-6

Lasek RJ, Chren MM. Acne vulgaris and the quality of life of adult dermatology patients. Arch Dermatol 1998; 134:454-8.

Vexiau P, Husson C, Chivot M, Brerault JL, Fiet J, Julien R, et al. Androgen Excess in Women with Acne Alone Compared with Women with Acne and/or Hirsutism. J Invest Dermatol 1990; 94:279-328.

Goulden V, Stables GI, Cunliffe WJ. Prevalence of facial acne in adults. J Am Acad Dermatol 1999; 41:577-80.

Cogen AL, Nizet V, Gallo RL. Skin microbiota: a source of disease or defence? Br J Dermatol 2008; 158(3):442-55.

Johnson BA, Nunley JR. Topical therapy for acne vulgaris. How do you choose the best drug for each patient? Postgrad Med 2000; 107:69-70.

Roth RR, James WD. Microbial ecology of the skin. Annu Rev Microbiol 1988; 42:441-64

Ghodsi SZ, Orawa H and Zouboulis CC. Prevalence, severity, and severity risk factors of acne in high school pupils: A community-based study. J Invest Dermatol 2009; 129:2136-41.

Tan HH, Tan AW, Barkhman T, Yan XY and Zhu M. Community of acne vulgaris in aldoscents in Singapore. Br J Dermatol 2007; 157:547-51.

Zandi S, Vares B, Abdollahi H. Determination of microbial agents of acne vulgaris and Propionibacterium acnes antibiotic resistance in patients referred to dermatology clinics in Kerman, Iran,. Jundishapur J Microbiol 2011; 4(1):17-22

Al-Zoman YA, Facharizt A, Al-Asmari KA. Pattern of skin diseases at Riyadh Military Hospital. Egyptian Dermatol J 2008; 4(2):4.

Kriger JN, Kauser DL and Wenzel RP. Nosocomial infections cause infections in acne patients. Surgery 1993; 156:313-16.

Ikaraoha CI, Taylor GL, Anetor JI, Ukaegbu QO, Nwobu GO. Demographic features, beliefs and socio-phychological impact of acne vulgaris among its sufferes in two towns in Nigeria. OJHAS 2009; 2:15-20

Hafez. AM, Mahran MA, Mohammed AK, Mohammed RE, Darweesh MA, Aal AA. Quality of Life in Acne Vulgaris Patients. J Neurol Psychiat 2007; 44(1):301-12.

Yu SY, Cheng WY, Chen CW. Lifetime Course of Acne: A Retrospective Questionnaire Study in School Teachers. Dermatol Sinica 2008; 26: 10-15

Sharquie EK, Al-Hamdi IK, Adil A, Noaimi AA, Al-Battat AR. Scarring and None Scarring Facial Acne Vulgaris and the Frequency of associated Skin Diseases. J Med 2009; 8(4):13-33

Biswas S, Mondal KK, Saha I, Dutta NR, Lahiri KS. Clinico-epidemiological Features of Acne Vulgaris: A Tertiary Hospital-Based Study. Iran J Dermatol 2010; 13(2):37-41

Hassanzadeh P, Bahmani M, Mehrabani D. Bacterial resistance to antibiotics in acne vulgaris: an in vitro study. Indian J Dermatol 2008; 53(3):122-4.

Cunliffe WJ. Management of adult acne and acne variants. J Cutan Med Surg 1998; 2(3):7-13.

Nakatsuji T, Liu YT, Huang CP, Gallo RL, Huang CM. Antibodies elicited by inactivated Propionibacterium acnes-based vaccines exert protective immunity and attenuate the IL-8 production in human sebocytes: relevance to therapy for acne vulgaris. J Invest Dermatol 2008; 128:2451-7.

Biatecka A, Mak M, Biedroñ R, Bobek M, Kasprowicz A, Marcinkiewicz J. Different pro-inflammatory and immunogenic potentials of Propionibacterium acnes and Staphylococcus epidermidis: implication for chronic inflammatory acne. Arch Immunol Ther Exp 2005; 53:79-85

Nielsen ML, Raahave D, Stage JG, Justesen T. Anaerobic and aerobic skin bacteria before and after skin-disinfection with chlorhexidine: An experimental study in volunteers. J Clin Path 1975; 28:793-7.

Hatemi G, Bahar H, Uysal S, Mat C, Gogus F, Masatlioglu S, et al. The pustular skin lesions in Behc¸et’s syndrome are not sterile. Ann Rheum Dis 2004; 63:1450-2.

Bojar RA, Holland KT. Acne and Propionibacterium acnes. Clin Dermatol 2004; 22:375-9.

Gollnick H. Management of acne. A report of the global alliance to improve outcomes in acne. J Am Acad Dermatol 2003; 49:1-38.

Leeming JP, Holland KT, Cunliffe WJ. The microbial ecology of pilosebaceous units isolated from human skin. J Gen Microbiol 1984; 130:803-7.

Lacaz CS, Heins-Vaccari EM, Hernandez-Arriagada GL, Martins EL, Prearo CL, Corim, SM et al. Primary cutaneous cryptococcosis due to Cryptococcus neoformans var. gattii serotype B, in an immunocompetent patient. Rev Inst Med Trop Sao Paulo 2002; 44(4):225-8.

Knoke M, Schwesinger G. One hundred years ago: the history of cryptococcosis in Greifswald. Medical mycology in the nineteenth century 1994; 37(8):229-33.

Hazen KC, Howell SA. Candida, cryptococcus and other yeasts of medical importance. Manual of Clinical Microbiology 2007; 9:1762-88.

Zouboulis C, Eady A, Philpott M, Goldsmith L, Orfanos C, Cunliffe WJ. What is the pathogenesis of acne. Exp Dermatol 2005; 14:143-52.

Tallab TM. Beliefs, perceptions and psychological impact of acne vulgaris among patients in the Assir region of Saudi Arabia. West Afr J Med 2004; 23:85-7.




How to Cite

Muhammed, N. I., & Dabbagh, R. A. (2016). Isolation and identification of microorganisms in acne patients. Zanco Journal of Medical Sciences (Zanco J Med Sci), 20(2), 1330–1336.



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